重庆潼南朱砂叶螨田间抗性监测及其抗性机制分析

本研究通过生物测定检测了重庆潼南田间朱砂叶螨种群对5种杀虫杀螨剂的抗药性,并测定了主要代谢酶活性及抗性相关基因的表达量。抗性监测结果显示,朱砂叶螨对联苯肼酯和哒螨灵表现为中等水平抗性,对阿维菌素、丁氟螨酯和甲氰菊酯为低水平抗性。代谢酶活性检测结果显示:田间种群的P450和GST活性明显提高,且多个相关基因的表达也显著上调,而CarE的活性没有显著变化。综上所述,重庆潼南田间朱砂叶螨种群已对联苯肼酯、哒螨灵、阿维菌素、丁氟螨酯和甲氰菊酯产生了不同程度的抗药性,P450和GST活性提高和有关基因过表达是抗性产生的主要原因。

Resistance monitoring and mechanism analysis of Tetranychus cinnabarinus collected from the field in Tongnan, Chongqing

The resistance of Tetranychus cinnabarinus from the field in Tongnan, Chongqing to 5 pesticides was tested through bioassay. The activity of detoxification enzymes and the relative expression of related genes were analyzed. The results showed that the field population of T. cinnabarinus had developed middle-level resistance to bifenazate and pyridaben, and low-level resistance to abamectin, cyflumetofen and fenpropathrin. Detoxification enzyme analysis indicated that the enzyme activity and gene expression of P450 and GST were both increased in the field population, but those of CarE did not change significantly. In conclusion, field populations of T. cinnabarinus in Tongnan had developed different resistance levels to bifenazate, pyridaben, abamectin, cyflumetofen and fenpropathrin. The resistance was mainly induced by the increased activity of detoxification enzymes (P450, GST) and over-expression of related genes.