| 套式PCR直接检测印度腥黑穗病菌冬孢子 |
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| 引用本文： | 易建平,陶庭典,潘良文,沈禹飞,印丽萍,郑建中. 套式PCR直接检测印度腥黑穗病菌冬孢子[J]. 植物检疫, 2002, 16(4): 197-200 |
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| 作者姓名： | 易建平 陶庭典 潘良文 沈禹飞 印丽萍 郑建中 |
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| 作者单位： | 上海出入境检验检疫局,200135 |
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| 基金项目： | 国家检验检疫局课题“小麦印度腥黑穗病菌鉴定检测技术”的一部分 |
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| 摘 要： | 用印度腥黑穗病菌冬孢子制备模板DNA ,利用印腥特异性引物T3 /T6,T3 /T4和套式PCR(nestPCR)扩增技术直接检测印腥冬孢子 ,检测的灵敏度可达 1个冬孢子。检测时间缩短为 1天。这种简单、快速、灵敏、实用和准确的PCR检测技术适用于口岸印腥检疫的需要 ,解决了常规PCR检测中DNA制备需要萌发冬孢子和检测时间长的难题。
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| 关 键 词： | 套式PCR 直接检测 印度腥黑穗病菌 冬孢子 |
Direct detection of Tilletia indica teliospores by nest PCR |
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| Yi Jianping,Tao Tingdian,Pan Lianwen,Shen Yufei,Yin Liping,Zheng Jianzhong. Direct detection of Tilletia indica teliospores by nest PCR[J]. Plant Quarantine, 2002, 16(4): 197-200 |
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| Authors: | Yi Jianping Tao Tingdian Pan Lianwen Shen Yufei Yin Liping Zheng Jianzhong |
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| Abstract: | A simple and practical nest polymerase chain reaction(PCR)method using primers T3/T4 and T3/T4 was modified to directly detect teliospores of Tilletia indica. One teliospores can be amplified and obtained specific product of 415bp.The process of detection was reduced to 8 hours.This method that overcome difficulties of dormancy of teliospore and extraction of mycelium DNA for PCR is potential in the detection of Tilletia indica. |
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| Keywords: | nest PCR direct detection Tilletia indica teliospore |
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