盐胁迫下番茄幼苗中不同性质蛋白表达差异的研究

[目的]分析不同盐浓度胁迫下番茄幼苗叶中不同性质蛋白表达的差异。[方法]以番茄种子为试材,置于附加不同盐浓度的1/2MS培养基上培养出番茄幼苗,然后分别提取其叶中的水溶蛋白、盐溶蛋白、酸溶蛋白、醇溶蛋白和总蛋白,并运用SDS-PAGE技术对其蛋白组成和表达量进行比较分析。[结果]不同盐浓度处理下,叶片蛋白质的组成和数量均发生变化,高盐浓度时多出了66.2 kD左右的蛋白质,说明在高盐浓度胁迫下植物产生了某些抗逆性蛋白;总蛋白条带比较相似,总蛋白含量和盐溶蛋白含量略微减少,水溶蛋白含量略微增加,并且在盐溶蛋白中增加了1条带,这些蛋白变化可能是盐胁迫下应激反应的相关蛋白。[结论]该研究为植物的抗病性选育提供一定的理论基础。

Study on Expression Difference of Protein with Different Properties in Tomato Seedlings under Salt Stress

[Objective] The study aimed to analyze the expression difference of protein with different properties in tomato seedlings under different salt stress.[Method] With the tomato seeds as the materials,they were put on the 1/2MS medium with different salt concn.and cultured into the tomato seedlings,and then the water soluble protein,salt soluble protein,acid soluble protein,alcohol soluble protein and total protein were extracted from the tomato seedlings resp.and their protein composition and expression amount were compared and analyzed by SDS-PAGE technique.[Result] Under the stress treatments with different salt concn.,the protein composition and amount in the leaf protein all varied,about 66.2 kD extra protein was got at high salt concn.,showed that the plant could produce some stress-resistance proteins under high salt concn.stress;the bands of total protein were relatively similar,the contents of total protein and salt soluble protein were slightly decreased,the water soluble protein was slight increased,and a band was increased in the salt soluble protein,showing that the change of the proteins might be the related proteins for the stress response to the salt stress.[Conclusion] The study provided some theoretical basis for the disease-resistance breeding of plant.