| 抗生素对青香蕉苹果叶片再生的影响 |
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| 引用本文： | 刘静,张元湖,刘庆忠,高玉娇.抗生素对青香蕉苹果叶片再生的影响[J].山东农业科学,2009(7):53-55. |
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| 作者姓名： | 刘静 张元湖 刘庆忠 高玉娇 |
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| 作者单位： | 1. 山东农业大学生命科学学院/作物生物学国家重点实验室,山东,泰安,271018
2. 山东省果树研究所,山东,泰安,271000 |
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| 基金项目： | 山东省自然科学基金项目 |
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| 摘 要： | 以青香蕉苹果组培苗幼叶为试材，研究了卡那霉素、头孢霉素和羧苄青霉素对青香蕉苹果叶片不定芽再生的影响。用继代10天左右的新梢顶部新展开叶片作为外植体，分别接种在MS＋2．0mg／LBA＋0．3mg／LTDZ＋0．8mg／L IAA＋30．0g／L蔗糖＋6．0g／L琼脂的再生培养基上，添加不同浓度的抗生素。结果表明：5mg／L卡那霉素极显著地抑制青香蕉苹果叶片不定芽再生，600mg／L头孢霉素和400mg／L羧苄青霉素均能完全抑制叶片不定芽再生。
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| 关 键 词： | 青香蕉苹果 离体叶片 抗生素 |
Effects of Antibiotics on Leaf Regeneration of Apple Cultivar White Winter Pearmain |
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| LIU Jing,ZHANG Yuan-hu,LIU Qing-zhong,GAO Yu-jiao.Effects of Antibiotics on Leaf Regeneration of Apple Cultivar White Winter Pearmain[J].Shandong Agricultural Sciences,2009(7):53-55. |
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| Authors: | LIU Jing ZHANG Yuan-hu LIU Qing-zhong GAO Yu-jiao |
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| Institution: | 1.College of Life Science;Shandong Agricultural University/State Key Laboratory of Crop Biology;Taian 271018;China;2.Shandong Institute of Pomology;Taian 271000;China |
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| Abstract: | With the young leaves of apple cv.White Winter Pearmain as materials,the effects of kanamycin,cefotaxime and carbenicillin on the regeneration of adventitious buds were studied.The leaves on the top of shoots after 10-day subculture were cultured on the MS medium containing 2.0 mg/L BA,0.3 mg/L TDZ,0.8 mg/L IAA,30.0 g/L sucrose and 6.0 g/L agar added several kinds of antibiotics.The results showed that 5 mg/L kanamycin could very significantly inhibit the regeneration of adventitious shoots,while 600 mg/L c... |
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| Keywords: | Apple cv White Winter Pearmain Leaves in vitro Antibiotic |
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