Identification of anthocyanins in Rhamnus alaternus L. berries |
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Authors: | Longo Luigia Vasapollo Giuseppe Rescio Leonardo |
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Affiliation: | Dipartimento di Ingegneria dell'Innovazione, Università di Lecce, via per Arnesano km 1, 73100 Lecce, Italy. |
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Abstract: | Anthocyanin composition in berries of Rhamnus alaternus L., a perennial wild shrub typical of the Mediterranean area, was determined for the first time. The pigments were extracted from the berries with 0.1% HCl in methanol and purified using a C-18 solid-phase cartridge. High-performance liquid chromatography diode array detection-mass spectrometry analysis showed that delphinidin 3-O-rutinoside represented about 62.4% of the total pigments. Other anthocyanins were 3-O-rutinoside derivatives of cyanidin (8.4%), petunidin (15.8%), pelargonidin (4.7%), and peonidin and malvidin (8.7%). The concomitant presence of the six most common anthocyanidins suggested that R. alaternus berries, besides being a good pigment source, could also be a useful tool for anthocyanin identification. |
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