高钙饲粮对青年蛋鸡血清一氧化氮浓度和抗氧化功能的影响

本试验旨在研究高钙饲粮对青年蛋鸡血清一氧化氮（ＮＯ）浓度和抗氧化功能的影响。将 ３５日龄 １００羽伊沙蛋鸡随机均分为对照组（含钙 １．００％）和高钙组（含钙 ３．７８％）,每组 ５个重复,每个重复 １０羽。每天观察鸡的精神状态、食欲和粪便情况。在饲喂高钙饲粮第 ０、８、１６、２４和 ３２天时,分别测定 ２组鸡的血清中 ＮＯ浓度和丙二醛含量及抗氧化酶系统的变化。结果表明：１）高钙饲粮能引起青年蛋鸡长期腹泻,在饲喂高钙饲粮的第 ８天就发生高血钙症。２）与对照组相比,高钙组在饲喂高钙饲粮第 ８天和第 １６天血清 ＮＯ浓度无显著变化（Ｐ＞０．０５）,第２４天和第 ３２天显著升高（Ｐ＜０．０５）;第 ８天和第 ２４天血清超氧化物歧化酶活性、总抗氧化能力无显著变化（Ｐ＞０．０５）,第 １６天显著升高（Ｐ＜０．０５）,第 ３２天显著降低（Ｐ＜０．０５）;第 ８、１６、２４天血清丙二醛含量无显著变化（Ｐ＞０．０５）,第 ３２天显著升高（Ｐ＜０．０５）;第 ２４天和第 ３２天黄嘌呤氧化酶活性有升高趋势（Ｐ＞０．０５）。结果提示,饲喂高钙饲粮可导致青年蛋鸡发生长期腹泻,引起高血钙症,导致其机体内自由基的产生与清除的动态平衡遭到破坏,使自由基的产生远远超出清除能力。

High Calcium Diet Affects Serum Nitric Oxide Concentration and Antioxidant Function of Pullets

This experiment was conducted to study the effects of high calcium diet on nitric oxide(NO) concentration and antioxidant function of pullets.One hundred healthy pullets aged 35 days were randomly divided into 2 groups with 5 replicates in each group and 10 chickens per replicate.The pullets in different groups were fed normal calcium diet(control group,1.00% calcium) and high calcium diet(HC group,3.78% calcium),respectively.Healthy situations of the pullets were inspected daily.NO concentration,malondialdehyde(MDA) content and enzymatic antioxidant activity in serum were detected on days 0,8,16,24 and 32 after feeding high calcium diet.The results showed as follows: 1) high calcium diet could cause diarrhea.Pullets would be hypercalcemia on day 8 after feeding high calcium diet.2) There was no significant difference in serum NO concentration of pullets between HC group and the control group on days 8 and 16 after feeding high calcium diet(P>0.05),but that in HC group was significantly increased on days 24 and 32 after feeding high calcium diet(P<0.05).There were no significant differences in serum superoxide dismutase(SOD) activity and total anti-oxidation capability(T-AOC) of pullets between HC group and the control group on days 8 and 24 after feeding high calcium diet(P>0.05),but those in HC group were increased on day 16 after feeding high calcium diet(P<0.05),meanwhile,decreased on day 32 after feeding high calcium diet compared with the control group(P<0.05).There was no significant difference in serum MDA content of pullets between HC group and the control group on days 8,16 and 24 after feeding high calcium diet(P>0.05),but that in HC group was significantly increased on day 32 after feeding high calcium diet(P<0.05).There was a increasing trend in serum xanthine oxidase(XOD) activity in HC group on days 24 and 32 after feeding high calcium diet compared with the control group(P>0.05).The results indicate that high calcium diet can cause long-term diarrhea,increase the lipid peroxidation,and decrease the enzymatic antioxidant activity.