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| 奶牛乳腺上皮细胞的二维和三维培养及培养后酪蛋白基因的表达 | |
| 引用本文： | 王秀美,侯先志,高爱武,考桂兰,高民,李大彪,兰儒冰,张楠,张艳明,黄清瑞. 奶牛乳腺上皮细胞的二维和三维培养及培养后酪蛋白基因的表达[J]. 动物营养学报, 2012, 24(6): 1157-1164 |
| 作者姓名： | 王秀美 侯先志 高爱武 考桂兰 高民 李大彪 兰儒冰 张楠 张艳明 黄清瑞 |
| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,呼和浩特,010018 2. 内蒙古农业大学食品科学与工程学院,呼和浩特,010018 3. 内蒙古农牧业科学院,呼和浩特,010010 |
| 基金项目： | 现代农业产业技术体系建设专项资金资助;内蒙古自然基金项目“奶牛乳腺上皮细胞三维培养体系的建立及其对酪蛋白合成的影响”(2010MS0406);国家973项目“牛奶重要营养品质形成与调控机理研究”(2011CB100803) |
| 摘 要： | 本试验研究了二维和三维培养对奶牛乳腺上皮细胞(BMEC)形态与β-酪蛋白、κ-酪蛋白、αs1-酪蛋白基因表达的影响.试验采用内蒙古地区3头5岁泌乳期健康荷斯坦奶牛的乳腺组织,将BMEC经3代培养纯化后分别进行二维和三维培养,观察不同培养模式下细胞的生长形态,测定β-酪蛋白、A-酪蛋白、αs1-酪蛋白基因的表达量.结果表明:2种培养模式下的BMEC呈现不同的细胞形态,二维培养的BMEC呈典型铺路石样细胞形态,三维培养的BMEC则形成了类腺泡的聚集物和管腔结构.利用三维培养的BMEC的αs1-酪蛋白基因的表达量显著地高于二维培养(P＜0.05),三维培养的BMEC的β-酪蛋白和κ-酪蛋白基因的表达量极显著地高于二维培养(P＜0.01).结果提示,三维培养的BMEC3种酪蛋白基因的表达量均高于二维培养,三维培养的BMEC在形态上呈现的特点与在体内更加接近. |
| 关 键 词： | 奶牛乳腺上皮细胞 二维培养 三维培养 酪蛋白 |
Two-and Three-Dimensional Cultivation of Bovine Mammary Epithelial Cells and Gene Expression of Casein after Cultured |
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| WANG Xiumei , HOU Xianzhi , GAO Aiwu , KAO Guilan , GAO Min , LI Dabiao , LAN Rubing , ZHANG Nan , ZHANG Yanming , HUANG Qingrui. Two-and Three-Dimensional Cultivation of Bovine Mammary Epithelial Cells and Gene Expression of Casein after Cultured[J]. CHINESE JOURNAL OF ANIMAL NUTRITION, 2012, 24(6): 1157-1164 | |
| Authors: | WANG Xiumei HOU Xianzhi GAO Aiwu KAO Guilan GAO Min LI Dabiao LAN Rubing ZHANG Nan ZHANG Yanming HUANG Qingrui |
| Affiliation: | 1(1.College of Animal Science and Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China; 2.College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China; 3.Inner Mongolia Academy of Agriculture and Animal Husbandry,Hohhot 010010,China) |
| Abstract: | This study was conducted to investigate the effects of two-and three-dimensional cultivation on cell morphology and gene expressions of β-casein,κ-casein and αs1-casein of bovine mammary epithelial cells(BMECs).Mammary parenchymal tissue was obtained from three healthy Holstein dairy cows aged 5 years.BMECs were purified after cultured for three generations,and then were cultured in two-and three-dimensional system,respectively.Cell morphology and gene expression levels of αs1-casein,β-casein and κ-casein of BMECs cultured in the two systems were compared.The results showed as follows: BMECs cultured in different systems displayed different cell morphologies,and those by two-dimensional cultivation displayed the classical cobblestone morphology,while those by three-dimensional cultivation formed mammospheres-forming aggregates and tubular structures.Gene expressions of αs1-casein(P<0.05),β-casein(P<0.01) and κ-casein(P<0.01) of BMECs by three-dimensional cultivation were significantly higher than those by two-dimensional cultivation.The results indicate that gene expressions of the three caseins of BMECs by three-dimensional cultivation are higher than those by two-dimensional cultivation,and morphological characteristics of three-dimensional cultured BMECs are closely similar to those in vivo. |
| Keywords: | bovine mammary epithelial cells two-dimensional cultivation three-dimensional cultivation casein |
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