土壤硝态氮的多波长紫外分光光度测定法

在大批量样本进行硝态氮分析,而且样本中硝态氮含量变化幅度较大时,工作量很大。我们在210nm 紫外分光光度法的基础上,扩大采用紫外波段多波长法进行测量,可省时省工,测定结果可达到酚二磺酸法相同甚至稍优的精确度。根据对干扰因子的分析,确定了多波长紫外分光光度法的操作步骤,并且已在研究施铵态氮肥土壤中的硝化作用时,应用本法进行大批量硝态氮的含量测定,取得有意义的结果。

STUDY ON THE MULTI-WAVELENGTH U-V SPECTROPHOTOMETRIC DETERMINATION ON SOIL NITRATE CONTENT

The analytical work of soil nitrate was time consumptive,when the variance of nitrate content were broad.Based on the 210 nm U-V spectrophotometric method,we designed a new multi-wavelength U-V spectrophotometric method to determine the soil nitrate. After comparison of multi-U-V method and routine method,it was demonst- rated that this method was a rapid and simply.It may shortened the detection time and decrease labour power input during the detection significantly,On the other hand,the result and the accuracy of detection data was also satisfactory. It was similar or even more accurate than the data detected by routine method- Phenol disulfonnic acid method. According the analysis to the results of disturbing factors such as Fulvic acid and other metal ions which influence the detection accuracy,we designed the detection procedure of multi-U-V method in detail.Owing to verify the feasibility of this method in the practical field,we had also applied this detection procedure to research work.