葡萄糖酸钠对光滑球拟酵母CCTCC M202019能量代谢和丙酮酸积累的影响

在好氧条件下,光滑球拟酵母(Torulopsis glabrata)CCTCC M202019能够利用葡萄糖酸钠为唯一碳源生长并积累少量丙酮酸。在含有90 g/L葡萄糖的培养基中,16 h时添加10 g/L葡萄糖酸钠,与100 g/L葡萄糖作为唯一碳源的对照组相比,细胞质量浓度、葡萄糖消耗速率、丙酮酸生产强度分别提高7.96%、13.04%和32.81%;其原因在于葡萄糖酸钠的添加,使胞内NAD+浓度提高了29.73%,NADH/NAD+比值和ATP含量分别下降26.89%和8.50%,此变化显著缓解了NADH和ATP对于糖酵解途径的抑制作用,从而显著加快糖酵解速率。

Effect of Sodium Gluconate on Pyruvate Accumulation by Torulopsis glabrata CCTCC M202019

The dry cell weight of T.glabrata reached to 3.5 g/L when 30 g/L of gluconate was presented in the culture medium.When 10 g/L of gluconate was supplemented to the culture broth with 90 g/L of glucose at 16 h,the dry cell weight,the rate of glucose consumption and the pyruvate production were increased by 7.96%,13.04% and 32.81%,respectively.The enhancement of glucose consumption and pyruvate production rate were due to the fact that the concentration of NAD+increased by 29.73% while the concentration of ATP and the ratio of NADH/NAD+ decreased 26.89% and 8.50%,respectively.The decrease in the intracellular NADH and ATP level decreased the inhibition effect of NADH and ATP,thus significantly increased the glycolytic ratio.