| α-淀粉酶产生菌的分离筛选与诱变选育 |
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| 引用本文： | 刘雅琴,陈海魁,孔令全.α-淀粉酶产生菌的分离筛选与诱变选育[J].畜牧与饲料科学,2010,31(9). |
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| 作者姓名： | 刘雅琴 陈海魁 孔令全 |
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| 作者单位： | 北方民族大学生物科学与工程学院; |
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| 基金项目： | 国家民委发酵酿造工程生物技术重点实验室资助项目 |
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| 摘 要： | 从土样、水样和面样中分离产α-淀粉酶的芽胞杆菌,对3个样品进行淀粉平板分离和革兰氏染色、芽胞染色,选出产α-淀粉酶芽胞杆菌10株(土样4株、面样3株、水样3株).同时测得其水解圈直径与菌落直径的比值,均在1.9～2.6之间,通过产淀粉酶液体培养基发酵产酶,采用比色法测定发酵液中α-淀粉酶活力均在4.8～5.7 g/mL之间.以筛选出产酶活力较高的菌株为出发菌株,进行紫外线诱变选育,经过大量筛选得到优良菌株,在适宜条件下经过发酵其α-淀粉酶活力可达9.6～13.0g/mL,较出发菌株酶活力提高了69.9%～129.5%.
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| 关 键 词： | 产淀粉酶 芽胞杆菌 分离 筛选 诱变 |
Separation and Mutagenesis of Alpha-amylase Producing Bacillus |
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| LIU Ya-qin,CHEN Hai-kui,KONG Ling-quan.Separation and Mutagenesis of Alpha-amylase Producing Bacillus[J].Animal Husbandry and Feed Science,2010,31(9). |
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| Authors: | LIU Ya-qin CHEN Hai-kui KONG Ling-quan |
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| Institution: | LIU Ya-qin,CHEN Hai-kui,KONG Ling-quan(School of Biological Science and Engineering,Northern University for Nationalities,Yinchuan 750021,China) |
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| Abstract: | Ten alpha-amylase producing Bacillus strains were isolated from soil (four strains), water (three strains) and flour (three strains) through isolation with starch plates, Gram's staining and spore staining.The ratio of hydrolysis zone diameter to colony diameter was 1.9-2.6.Liquid culture medium was used for fermentation to produce amylase.The activity of alpha-amylase was determined bycolorimetry, and it was 4.8-5.7 g/mL in fermentation broth.The original strains producing high-activity alpha-amylase was s... |
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| Keywords: | amylase production Bacillus separation selection mutagenesis |
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