| 菊花花瓣的组织培养技术研究 |
| |
| 引用本文： | 李平,李庆伟,贺爱莉,樊亚敏,孙新政. 菊花花瓣的组织培养技术研究[J]. 北方园艺, 2009, 0(9) |
| |
| 作者姓名： | 李平 李庆伟 贺爱莉 樊亚敏 孙新政 |
| |
| 作者单位： | 河南农业职业学院,植物科学系,河南,中牟,451450 |
| |
| 摘 要： | 以紫色线状菊花花瓣为外植体,研究了不同培养基组成、外植体摆放方式、光照条件对其再生的影响.结果表明:菊花花瓣诱导适宜光照时间是12～16 h/d;花瓣花背向上比花背向下效果不定芽的诱导效果好,而在愈伤组织诱导时花背向下比花背向上效果好;MS+NAA 1.0 mg/L或2,4-D 1.0 mg/L+6-BA 1.0 mg/L可作为外植体的愈伤组织诱导的培养基;MS+6-BA 1.0 mg/L能直接从花瓣上诱导产生不定芽;MS+IBA 0.2 mg/L+6-BA 1.5 mg/L丛生芽诱导率高,且产生的丛生芽质量高;1/2MS+IBA 0.3 mg/L为适宜的生根培养基.
|
| 关 键 词： | 菊花 花瓣 愈伤组织 丛生芽 植株再生 |
Studies on Petal Tissue Culture of Chrysanthemum morifolium |
| |
| LI Ping,LI Qing-wei,HE Ai-li,FAN Yan-min,SUN Xin-zheng. Studies on Petal Tissue Culture of Chrysanthemum morifolium[J]. Northern Horticulture, 2009, 0(9) |
| |
| Authors: | LI Ping LI Qing-wei HE Ai-li FAN Yan-min SUN Xin-zheng |
| |
| Affiliation: | Henan Vocational College of Agriculture;Zhongmu;Henan 451450;China |
| |
| Abstract: | The purple petal of Chrysanthemum morifolium were used as explants,studied the different culture medium composition,explants and light to petal regeneration influence.The results showed that petal induction being suitable light time was 12~16 h/d;The Petal Normal place of adventitious buds induced was better.Yet.The callus induction and the results of the contrary.MS+NAA 1.0 mg/L or 2,4-D 1.0 mg/L+6-BA 1.0 mg/L can be used as explants of callus induction medium;MS+6-BA 1.0 mg/L directly from the petals on t... |
| |
| Keywords: | Chrysaruhemnn morifolium Petal Callus Problems bud Plant regeneration |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
