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| 湖南省鸡蛔虫线粒体nad4基因序列的测定及分析 | |
| 引用本文： | 李淼,蔡丝丝,姚明锐,刘升,李许梅,侯强红.湖南省鸡蛔虫线粒体nad4基因序列的测定及分析[J].现代农业科技,2012(4):321+325. |
| 作者姓名： | 李淼 蔡丝丝 姚明锐 刘升 李许梅 侯强红 |
| 作者单位： | 1. 环化职业技术学院,湖南怀化418000／湖南农业大学动物医学院 2. 湖南农业大学动物医学院 3. 环化职业技术学院,湖南怀化,418000 |
| 摘 要： | 为阐明鸡蛔虫湖南分离株线粒体烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位4(nad4)基因部分序列(pnad4)遗传变异情况,利用聚合酶链式反应(PCR)扩增鸡蛔虫的pnad4,并对序列进行比对。结果表明:所获得的pnad4序列长度均为408 bp。由于鸡蛔虫pnad4序列种内相对保守,种间差异较大,故可作为种间遗传变异研究的标记,从而为鸡蛔虫的分类鉴定及进一步的分子流行病学调查奠定基础。 |
| 关 键 词： | 鸡蛔虫 线粒体DNA pnad基因 序列分析 |
Sequencing and Analyzing on Mitochondria Gene nad4 of Ascaridia galli Isolated from Hunan Province |
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| Authors: | LI Miao CAI Si-si YAO Ming-rui LIU Sheng LI Xu-mei HOU Qiang-hong |
| Institution: | 1 *(1 Huaihua Vocational and Technical College,Huaihua Hunan 418000;2 College of Veterinary Medicine,Hunan Agricultural University) |
| Abstract: | For elaborating genetic variation,the subunit gene nad4 of NADH of Ascaridia galli isolated from Hunan Province was sequenced and analyzed.PCR amplification which amided at part of nad4 gene(pnad4)had been performed,and the amplicons had been compared to other sequences stored at GenBank.Results revealed that all the size of the amplicons were 408 bp.Due to the conservation of pnad4 gene was only in the same specie,it had been thought to be genetic marker.This study had laid the foundation for identification and molecular epidemiology investigation of Ascaridia galli. |
| Keywords: | Ascaridia galli Mitochondrial DNA pnad4 gene sequence analysis |
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