| 紫花苜蓿肽基脯氨酰顺反异构酶基因全长cDNA的电子克隆及同源建模 |
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| 引用本文： | 王海波.紫花苜蓿肽基脯氨酰顺反异构酶基因全长cDNA的电子克隆及同源建模[J].安徽农业科学,2010,38(8):3925-3927. |
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| 作者姓名： | 王海波 |
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| 作者单位： | 曲靖师范学院生物资源与环境科学学院,云南曲靖,655011 |
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| 基金项目： | 曲靖师范学院校内青年科研基金项目(2008QN031) |
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| 摘 要： | 目的]研究对紫花苜蓿肽基脯氨酰顺反异构酶基因全长cDNA进行电子克隆及同源建模的方法。方法]根据物种间同源基因相对保守的特点,利用NCBI数据库,对公共EST数据库进行同源检索筛选和重叠群装配,获得拼接cDNA序列,经过ORFFinder分析后获得相应蛋白质的全长序列,并利用Swiss-Mode服务器预测各原子坐标,进而利用Swiss-PdbViewer生成三维空间模型。结果]采用该方法成功获得紫花苜蓿肽基脯氨酰顺反异构酶基因的完整cDNA序列,全长为1880bp,mPPI蛋白质编码68AA,分子量约为7829.9Da,理论等电点pI=8.99,原子组成为C338H547N103O103S4,摩尔消光系数280nm处为4595,不稳定系数为42.16,脂肪系数为83.24,总平均亲水性为-0.456。结论]根据物种间同源基因序列对跨物种间EST数据库进行同源检索筛选和拼接,是基因克隆的一条有效途径。
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| 关 键 词： | 紫花苜蓿 肽基脯氨酰顺反异构酶(PPI) 电子克隆 聚类分析 |
Full-length cDNA in Silico Cloning and Homology Modeling of Gene Encoding Peptidyl Prolyl Cis-trans Isomerase from Medicago sativa L. |
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| WANG Hai-bo.Full-length cDNA in Silico Cloning and Homology Modeling of Gene Encoding Peptidyl Prolyl Cis-trans Isomerase from Medicago sativa L.[J].Journal of Anhui Agricultural Sciences,2010,38(8):3925-3927. |
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| Authors: | WANG Hai-bo |
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| Institution: | College of Biological Resource and Environmental Science;Qujing Normal University;Qujing;Yunnan 655011 |
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| Abstract: | Objective] The study aimed to research the method of making full-length cDNA in silico cloning and homology modeling of the gene encoding peptidyl prolyl cis-trans isomerase (mPPI) from Medicago sativa L.Method] According to the characters that the homology genes were relatively conservative among species,the public ESTs database from NCBI related to mPPI were searched and screened,and the associated ESTs were made for assembly and then the joined total cDNA sequence was obtained.The full-length sequence ... |
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| Keywords: | Medicago sativa L Peptidyl prolyl cis-trans isomerase(PPI) In silico cloning Homology modeling |
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