| SSR对甜菜骨干单胚雄性不育系和保持系的遗传多样性分析 |
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| 引用本文： | 王茂芊,马龙彪,吴则东,王华忠.SSR对甜菜骨干单胚雄性不育系和保持系的遗传多样性分析[J].中国农学通报,2015,31(32):62-67. |
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| 作者姓名： | 王茂芊 马龙彪 吴则东 王华忠 |
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| 作者单位： | (;1.黑龙江省普通高校甜菜遗传育种重点实验室/黑龙江大学,哈尔滨 150080；;2.中国农业科学院甜菜研究所/黑龙江大学农作物研究院,哈尔滨 150080；;3.中国农业科学院北方糖料作物资源与利用重点开放实验室,哈尔滨 150080） |
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| 基金项目： | 国家自然科学基金项目“甜菜分子标记遗传图谱构建及重要农艺性状QTL分析”(31271780)；国家现代农业产业技术体系建设专项“东北区育种岗位专家”(CARS-210104)；黑龙江省普通高校甜菜遗传育种重点实验室开放课题“分子标记对甜菜不育系和保持系的亲缘关系分析”。 |
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| 摘 要： | 为了配制优良单胚杂交组合，避免基因组成重复，提高育种工作效率，利用14对SSR引物对41对甜菜骨干单胚不育系和保持系进行检测。结果表明，14对SSR引物共产生93条扩增带，其中多态性条带76条，平均每对引物扩增多态性条带5.4条，平均多态性百分比为81.7%，平均遗传距离为0.3572，平均遗传相似系数为0.6996。不同品系的平均遗传相似系数为混合(0.7549)>兰45-46(0.7513)>兰71-72(0.7493)>兰19-20(0.7465)>兰85-86(0.7337)。根据UPGMA方法进行聚类，在遗传距离0.20处，可将82份供试材料分为两大类群。结果表明，供试的甜菜单胚雄性不育系和保持系的遗传多样性较丰富，不同品系的不育系和保持系材料之间的遗传基础有差异。
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| 关 键 词： | 台风“达维” 台风“达维” 台风定位 路径分析 强度分析 |
| 收稿时间： | 2015/7/21 0:00:00 |
| 修稿时间： | 2015/7/29 0:00:00 |
Genetic Diversity Analysis of Major Male Sterile and Maintenance Monogerm Sugar Beet Line by SSR |
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| Wang Maoqian,Ma Longbiao,Wu Zedong and Wang Huazhong.Genetic Diversity Analysis of Major Male Sterile and Maintenance Monogerm Sugar Beet Line by SSR[J].Chinese Agricultural Science Bulletin,2015,31(32):62-67. |
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| Authors: | Wang Maoqian Ma Longbiao Wu Zedong and Wang Huazhong |
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| Institution: | (;1.Key Laboratory of Sugar Beet Genetic Breeding/Heilongjiang University, Harbin 150080;2.Sugar Beet Research Institute Chinese Academy of Agricultural Sciences/Crop Academy of Heilongjiang University, Harbin 150080;3.Key Laboratory of North Sugar Crop Resource and Utilization, Chinese Academy of Agricultural Sciences, Harbin 150080) |
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| Abstract: | In this study, 14 pairs of SSR molecule markers were used to investigate the relationship of 41 pairs of Major Male Sterile and Maintenance Monogerm Sugar Beet Line, in order to prepare the combination of the fine single embryo, avoid the duplication of gene composition and improve the efficiency of breeding. Results showed that, a total of 93 unambiguous bands were obtained, 76 of which were polymorphic. On average, each pair of primer amplified 5.4 genotypes. The average ratio of polymorphic bands was 81.7%. Compute over-all mean showed that genetic distance was 0.3572, genetic similarity among varieties was 0.6996. The genetic similarities within different groups of genotypes were, 0.7549 among mixed one, 0.7513 among LAN 45-46, 0.7493 among LAN 71-72, 0.7465 among LAN 19-20, and 0.7337 among LAN 85-86. The 82 genotypes were divided into two cluster groups based on cluster analysis (at intercept of 0.2). The genotypes from Major Male Sterile and Maintenance Monogerm Sugar Beet Line showed high level of genetic diversity. There was a definite difference among different Male Sterile and Maintenance Monogerm genetic background. |
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| Keywords: | sugar beet male sterile line of monogerm maintenance line SSR genetic diversity |
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