Saponin adjuvants. V. Precipitation of serum components by non-purified saponin adjuvants in agar gel diffusion. |
| |
Authors: | K Dalsgaard |
| |
Institution: | The State Veterinary Institute for Virus Research, Lindholm, DK-4771 Kalvehave, Denmark |
| |
Abstract: | The immunologically active adjuvant Quil A does not induce precipitating antibodies in rabbits. This was tested by immunodiffusion of serum samples taken after repeated injections of Quil A. Quil A does not react non-specifically with any of 6 different animal sera tested (rabbit, guinea pig, horse, sheep, cattle, and pig). Two commercially available saponins with known adjuvant activity (Saponin MT, E. Merck, and Saponin P 3, Food Industries) produced non-specific precipitation in double gel diffusion tests with all the sera, as did crude extracts of the South-American tree Quillaja saponaria Molina.}This phenomenon in relation to the local tissue damage caused by non-purified saponin preparations is discussed. |
| |
Keywords: | adjuvant saponin gel precipitation immunodiffusion Quil A |
|
|