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微卫星标记AY493302与缙云麻鸭蛋壳颜色的相关性分析
引用本文:袁青妍,陶争荣,李国勤,沈军达,陈奕春,陈文标. 微卫星标记AY493302与缙云麻鸭蛋壳颜色的相关性分析[J]. 中国农学通报, 2010, 26(8): 12-16
作者姓名:袁青妍  陶争荣  李国勤  沈军达  陈奕春  陈文标
作者单位:1. 浙江省农业科学院畜牧兽医研究所,杭州,310021
2. 缙云县畜牧兽医局,浙江缙云,321400
3. 浙江五莲农牧有限公司,浙江缙云,321403
基金项目:国家“十一五”科技支撑计划课题;浙江省重大科技攻关项目;浙江省重大国际合作项目
摘    要:采用50只青壳蛋缙云麻鸭及30只白壳蛋缙云麻鸭的血液DNA对微卫星标记AY493302进行多态性分析,探讨该微卫星标记与缙云麻鸭蛋壳颜色的相关性,为鸭青壳性状的分子标记辅助选择提供理论依据和技术支持。结果微卫星标记AY493302在青壳蛋与白壳蛋缙云麻鸭之间的基因型分布差异极其显著(P=2.247E-12),表明该微卫星标记与缙云麻鸭的蛋壳颜色高度相关。其中基因型JJ(365bp/365bp)不仅在2种鸭中的频率差异极其显著(P0.0001),而且只在青壳的缙云麻鸭中出现,在白壳的缙云麻鸭中尚未出现,表明基因型JJ(365bp/365bp)可以作为缙云麻鸭纯合青壳基因标记辅助选择的基因型。

关 键 词:蛋壳颜色
收稿时间:2009-10-22
修稿时间:2009-11-19

Analysis on Correlation Between Microsatellite Marker AY493302 and Eggshell Color of Jinyun Duck
Yuan Qingyan,Tao Zhengrong,Li Guoqin,Shen Jund,Chen Yichun,Chen Wenbi. Analysis on Correlation Between Microsatellite Marker AY493302 and Eggshell Color of Jinyun Duck[J]. Chinese Agricultural Science Bulletin, 2010, 26(8): 12-16
Authors:Yuan Qingyan  Tao Zhengrong  Li Guoqin  Shen Jund  Chen Yichun  Chen Wenbi
Affiliation:1Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021;2Jinyun Country Animal Husbandry and Veterinary Bureau, Jinyun Zhejiang 321400;3Zhejiang Wulian Agriculture and LivestockCorporation, Jinyun Zhejiang 321403
Abstract:Microsatellite locus AY493302 was used to analyze the polymorphisms of 50 blue-eggshelled and 30 white-eggshelled Jinyun ducks for the correlation relationship between this marker and eggshell color of Jinyun ducks. The results showed that the genotype distributions of this marker between two eggshell color ducks were significantly different (P=2.247E-12). This denotes that microsatellite AY493302 was highly correlated with eggshell color of Jinyun duck. The frequency of genotype JJ (365bp/365bp) was also significantly different between two eggshell color ducks (P<0.0001). Furthermore, this genotype existed only in blue-eggshelled Jinyun ducks, while not in white-eggshelled ones. These suggested that genotype JJ (365bp/365bp) can be used to select the homozygous blue-eggshelled gene of Jinyun duck in the form of assistant marker.
Keywords:eggshell colorzz
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