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黄波罗不同年龄材料微繁苗内源激素的比较分析
引用本文:李超峰,王力华,赵望峰,何晓刚,邱建.黄波罗不同年龄材料微繁苗内源激素的比较分析[J].辽宁林业科技,2007(1):16-19.
作者姓名:李超峰  王力华  赵望峰  何晓刚  邱建
作者单位:中国科学院,沈阳应用生态研究所,辽宁,沈阳,110016;中国科学院,研究生院,北京,100039;中国科学院,沈阳应用生态研究所,辽宁,沈阳,110016;新宾县陡岭林场,辽宁,新宾,113200
基金项目:中国科学院知识创新工程重要方向项目(SCX2-YW-N-031)
摘    要:不同年龄植物材料的植株再生能力不同,繁殖质量也不同,要提高繁殖效率和再生苗木的质量,就要深入了解初始材料的生理生化状态,以便为具有优良遗传特性的适龄材料选择提供依据。文章检测了不同年龄黄波罗组培苗的脱落酸、生长素、赤霉素、玉米素型细胞分裂素型和异戊烯基型细胞分裂素含量并做了统计学差异分析,结果表明:随试材年龄增长,黄波罗微繁苗的iP-type CKs/总Cks降低,而Z-type CKs/iP-type CKs和GAs含量增加且有显著的统计学差异,可作为选择适龄微繁材料的生理指标之一。

关 键 词:黄波罗  组织培养  内源激素
文章编号:1001-1714(2007)01-0016-04
修稿时间:2006-08-23

Analysis of Phase-change Related Endogenous Hormone Changes in Juvenile and Mature of Phellodendron amurense
LI Chao-feng,WANG Li-hua,ZHAO Wang-feng,HE Xiao-gang,QIU Jian.Analysis of Phase-change Related Endogenous Hormone Changes in Juvenile and Mature of Phellodendron amurense[J].Liaoning Forestry Science and Technology,2007(1):16-19.
Authors:LI Chao-feng  WANG Li-hua  ZHAO Wang-feng  HE Xiao-gang  QIU Jian
Institution:1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 2. Graduate School of Chinese Academy of Sciences, Beijing 100039,China;3.Xinbin County Douling Forestry Farm,Xinbin 113200,China
Abstract:The ability to propagation of woody plants with different age is different,whose quantitive indicies,could be used as a definte theoretical foundation of the selected material with age optium and to choose optimal conditions in order to improve forest programs and make them more profitable.In order to determine these hormonal indices,the contents of ABA,IAA,GAs,iP-type CKs and Z-type CKs were identified in cultured seedling of phellodendron amurense with different ages and analysed the statistical difference.The results showed that the ratio of iP-type CKs/total Cks decreased and Z-type CKs/ iP-type Cks and GAs increased throughout the whole developmental processes and had significantly statistical difference.The fact allowed us to consider the ratio of iP-type CKs/total Cks decreased and Z-type CKs/ iP-type Cks and GAs as the indices of the selected material with age optium.
Keywords:phellodendron amurense  tissue culture
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