Chlorophyll meter estimates leaf area‐based nitrogen concentration of rice

Abstract

The chlorophyll meter (SPAD‐502) can be used to diagnose the nitrogen (N) status of rice (Oryza sativa L) plants to determine the need for fertilizer‐N topdressing. However, accurate estimation of leaf N concentration on a dry weight basis (N_dw) by SPAD requires the adjustment of SPAD values for specific leaf weight (SLW). The objective of this study was to determine if SPAD estimates of leaf N concentration on a leaf area basis (Na) without correcting for SLW. SPAD values were measured from different genotypes at various growth stages with the chlorophyll meter. Leaf N concentration was determined by micro‐Kjeldahl procedure and expressed based on dry weight and leaf area. Specific leaf weight was calculated as the ratio of leaf dry weight to leaf area. At each growth stage, SPAD estimated Na better than Ndw, especially at the middle and later growth stages. When data for all stages were pooled, SPAD values also correlated with Na better than with Ndw (r value of 0.81 versus 0.43). This finding was also true across 80 genotypes. For estimation of N_a by SPAD, therefore, no adjustment of SPAD values for SLW is needed.