解淀粉芽胞杆菌B1619防控设施番茄枯萎病田间使用技术研究与示范

解淀粉芽胞杆菌B1619能够有效防控设施番茄枯萎病,研究菌株B1619田间高效使用技术对保证生防效果至关重要。本文对菌株B1619在接种番茄枯萎菌的病土中的定殖规律,菌株B1619水分散粒剂在田间的撒施方式和使用剂量,菌株B1619水分散粒剂对番茄枯萎病的田间防治效果进行了试验。结果表明,解淀粉芽胞杆菌B1619可以在接种枯萎菌的病土中稳定定殖并维持一定数量,在21 d时仍然有5.9×10~4 cfu/g土的种群数量。田间穴施和灌根的撒施方式防治效果明显好于蘸根和拌土,田间使用剂量应≥6 g/株。2年4地的田间药效试验结果表明,当田间撒施方式为穴施,田间使用剂量为32 kg/667 m~2时,菌株B1619水分散粒剂对番茄枯萎病的防治效果达到65.1%~85.2%,显著高于化学药剂百菌清。示范推广试验证明,菌株B1619水分散粒剂对番茄枯萎病的田间防治效果达到85%~90%。

Application Technologies and Demonstration Trials in Fields of Bacillus amyloliquefaciens B1619 against Tomato Fusarium Wilt

Bacillus amyloliquefaciens B 1619 possesses biocontrol activity against Fusarium wilt of tomato.It's essential to establish the high-efficiency application technology system under different environmental conditions.Colonizing ability,the appropriate dosage,usage and control efficiency of B1619 were studied in field trials.According to the results,the colonization in rhizosphere soil was up to 5.9 × 104 cfu/g,which reached the peak at 8th day and kept high level till 21th day.The control efficiency (65.1％-85.2％) was significantly higher than the chlorolthalonil (75％) when the fertilization methods were root-drenching or hole fertilization at a dose of greater than or equal to 6 g per plant.The demonstration trials indicated that the control efficiency of B 1619 against Fusarium wilt of tomato was up to 85％-90％.