丁香假单胞菌对亚洲玉米螟幼虫抗寒能力的影响

本文用丁香假单胞菌Pseudomonas syringae处理亚洲玉米螟Ostrinia furnacalis(Guenée)幼虫,使用过冷却仪测定过冷却点,分析该菌株对参试幼虫耐寒能力的影响。结果表明,亚洲玉米螟5龄非滞育幼虫抗寒能力比其他龄期非滞育幼虫强。亚洲玉米螟滞育5龄幼虫抗寒能力比非滞育5龄幼虫强。使用丁香假单胞菌处理亚洲玉米螟5龄非滞育和滞育幼虫,24 h后非滞育幼虫的过冷却点和结冰点分别提高了5.14和2.83℃。5龄滞育幼虫过冷却点和结冰点分别提高了11.25和4.65℃。丁香假单胞菌菌悬液(浓度为8×108 cfu/m L)对亚洲玉米螟具有较好的杀虫效果,非滞育5龄幼虫在(15±1)℃条件下处理24 h后平均死亡率39.1%。

Effect of Pseudomonas syringae on Cold Hardiness of Ostriniafurnacalis Larvae

Effect of Pseudomonas syringae on cold tolerance of the Asian corn borer larvae,Ostrinia furnacalis (Guenée),was analyzed by measureing the supercooling point.Results indicated that cold resistance of the non diapause 5th instar O.furnacalis larvae was stronger than that of other age groups.The cold resistance of the 5th instar diapause O.furnacalis larvae was higher than that of non diapause 5th instar larvae.When treated with Pseudomonas syringae for 24 h,the supercooling point and freezing point of non diapause 5th instar O.furnacalis larvae increased by 5.14 ℃ and 2.83 ℃,respectively;and diapause 5th instar larvae increased by 11.46 ℃ and 4.65 ℃,respectively.P syringae (at concentration of 8 × 108 cfu/ml) had a good insecticidal effect on O.furnacalis,with mortality rate of non-diapause 5th instar larvae being 39.1％ under 15 ℃ after 24 h.