| 陕西杨凌地区苹果树腐烂病菌（Valsa mali）基因多态性的SRAP分析 |
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| 引用本文： | 李正力,黄丽丽,康振生,臧 睿.陕西杨凌地区苹果树腐烂病菌（Valsa mali）基因多态性的SRAP分析[J].西北农业学报,2011,20(3):190-195. |
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| 作者姓名： | 李正力 黄丽丽 康振生 臧 睿 |
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| 作者单位： | 西北农林科技大学植物保护学院,陕西省农业分子生物学重点实验室,陕西,杨凌,712100 |
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| 基金项目： | 国家自然科学基金项目(30771396); 现代农业产业技术体系(苹果); 高等学校学科创新引智计划(B07049) |
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| 摘 要： | 利用SRAP标记对陕西省杨凌地区36株苹果树腐烂病菌(Valsa mali)基因多态性进行分析。从150对SRAP引物中筛选得到8对多态性高、稳定性好的引物组合对供试菌株进行扩增,共得到多态性条带56条,占总带数的75.68%,36株菌株的相似系数为0.339 6~0.962 2。其中,菌株167和165ZJG之间相似系数最大,亲缘关系最近。菌株SXYL24与SXYL135之间的相似系数最小。UPGMA聚类分析显示,在相似系数为0.846处36株供试菌株被划分为4个类群,与菌株采集来源及致病力不存在相关性。
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| 关 键 词： | 苹果树腐烂病菌 DNA多态性 相关序列扩增多态性 致病力 |
Polymorphism of Valsa mali Collected in Yangling Shaanxi Using SRAP Markers |
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| LI Zhengli,HUANG Lili,KANG Zhensheng and ZANG Rui.Polymorphism of Valsa mali Collected in Yangling Shaanxi Using SRAP Markers[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(3):190-195. |
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| Authors: | LI Zhengli HUANG Lili KANG Zhensheng and ZANG Rui |
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| Institution: | LI Zhengli,HUANG Lili,KANG Zhensheng and ZANG Rui (Plant Protection College,Northwest A&F University,Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling Shaanxi 712100,China) |
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| Abstract: | DNA polymorphism of 36 Valsa mali strains from Yangling,Shaanxi province was analyzed using 8 highly polymorphic and stable primer pairs screened from 150 pairs of SRAP primers.56 polymorphic bands were amplified,accounting for 75.68% of the total.The similarity coefficient of the 36 V.mali strains was 0.339 6-0.962 2.Strains 167 and 165ZJG had the maximum similarity coefficient and the closest relationship.With a genetic coefficient of 0.846,36 strains could be classified into 4 subgroups based on UPGMA cl... |
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| Keywords: | Valsa mali DNA polymorphism SRAP makers Pathogenesis |
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