| 川芎甜菜碱醛脱氢酶基因的克隆及序列分析 |
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| 引用本文： | 周嘉裕,廖 海.川芎甜菜碱醛脱氢酶基因的克隆及序列分析[J].西北农业学报,2011,20(3):45-51. |
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| 作者姓名： | 周嘉裕 廖 海 |
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| 作者单位： | 西南交通大学生命科学与工程学院,成都,610031 |
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| 基金项目： | 教育部中央高校基本科研业务费专项资金(SWJTU09ZT28,SWJTU09CX065); 西南交通大学“希望之星”与西南交通大学科技发展项目(2008B06); 中国科学院重点实验室开放课题 |
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| 摘 要： | 以川芎(Liqusticum churning Franch Hort)为材料,采用RT-PCR和RACE方法,从新鲜的嫩叶中克隆出甜菜碱醛脱氢酶(betaine aldehyde dehydrogenase,BADH)基因的cDNA序列。克隆到的cDNA序列全长为2 211 bp,编码一条由508个氨基酸残基组成的多肽,其核酸序列与人参的BADH基因的cDNA序列同源性为86%,对应编码氨基酸序列同源性为91%。将得到的序列提交GenBank,序列号为HM352764。与其他植物BADH的氨基酸序列比对,川芎BADH具有相同功能区域,包括N-端信号肽、底物结合及酶催化位点,因而可以参与甜菜碱的合成反应。对川芎BADH与其他植物BADH的氨基酸序列的进化分析表明,其与五加科人参的同源性较近。
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| 关 键 词： | 川芎 甜菜碱醛脱氢酶 克隆 序列分析 |
Molecular Cloning and Analysis of Betaine Aldehyde Dehydrogenase Gene of Liqusticum chuanxiong Franch Hort |
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| ZHOU Jiayu and LIAO Hai.Molecular Cloning and Analysis of Betaine Aldehyde Dehydrogenase Gene of Liqusticum chuanxiong Franch Hort[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(3):45-51. |
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| Authors: | ZHOU Jiayu and LIAO Hai |
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| Institution: | ZHOU Jiayu and LIAO Hai (College of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China) |
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| Abstract: | UsingLiqusticum churningFranch Hort as material,a cDNA of betaine aldehyde dehydrogenase(BADH) was cloned from tender leaves of Liqusticum churning by RT-PCR and RACE.The full-length cDNA of BADH had 2 211 bp with an open reading frame encoding 508 amino acids of protein.The accession number of BADH from Liqusticum churning in GenBank was HM352764.The BADH from Liqusticum churning had 86% nucleotide sequence homology and 91% amino acid sequence homology to the sequence of BADH from Panax ginseng,respectivel... |
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| Keywords: | Liqusticum churning Franch Hort Betaine aldehyde dehydrogenase Cloning Analysis of sequence |
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