| 用于PCR检测的扩展青霉基因组DNA提取方法比较 |
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| 引用本文： | 何鸿举,焦凌霞,樊明涛,刘晓娇,吕丽娟,魏新元.用于PCR检测的扩展青霉基因组DNA提取方法比较[J].西北农业学报,2011,20(2):198-201. |
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| 作者姓名： | 何鸿举 焦凌霞 樊明涛 刘晓娇 吕丽娟 魏新元 |
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| 作者单位： | 1. 西北农林科技大学,食品科学与工程学院,陕西杨凌,712100
2. 河南科技学院,食品学院,河南新乡,453003 |
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| 基金项目： | 教育部博士点基金(200807120017); 陕西省科技攻关项目(2007K01-12) |
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| 摘 要： | 为寻找一种经济、简便、高效的基因组DNA提取方法,进一步开展扩展青霉的分子生物学研究,采用氯化苄法、玻璃珠法、玻璃珠+氯化苄法、试剂盒法4种方法提取扩展青霉的基因组DNA,并测定DNA质量浓度以及进行PCR和电泳分析,比较不同提取方法的效果。结果表明,4种方法均可提取到基因组DNA,其抽提质量优劣依次为试剂盒法、玻璃珠+氯化苄法、玻璃珠法、氯化苄法。其中玻璃珠+氯化苄法提取效率接近试剂盒法,效果良好,并且此法成本低廉、操作简单、结果稳定,可代替昂贵的试剂盒法用于扩展青霉基因组DNA的提取。
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| 关 键 词： | 氯化苄法 玻璃珠法 DNA提取 PCR |
Comparison of Methods of Genomic DNA Extraction from Penicillium expansum for PCR Detection |
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| HE Hongju,JIAO Lingxia,FAN Mingtao,LIU Xiaojiao,L Lijuan,WEI Xinyuan.Comparison of Methods of Genomic DNA Extraction from Penicillium expansum for PCR Detection[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(2):198-201. |
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| Authors: | HE Hongju JIAO Lingxia FAN Mingtao LIU Xiaojiao L Lijuan WEI Xinyuan |
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| Institution: | HE Hongju1,JIAO Lingxia2,FAN Mingtao1,LIU Xiaojiao1,Lü Lijuan1 and WEI Xinyuan1(1.College of Food Science and Engineering,Northwest A&F University,Yangling Shaanxi 712100,China,2.College of Food Science,Henan Institute of Science and Technology,Xinxiang Henan 453003,China) |
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| Abstract: | The aim was to search an economic,convenient and high efficient extraction method for genomic DNA of Penicillium expansum to provide the scientific basis for further molecular biological study.The genomic DNA of Penicillium expansum strains were extracted by four methods such as Benzyl chloride,Glass beads,Glass beads with Benzyl chloride and ZR Fungal DNA Kit,and the effects of the different extraction methods were compared through the quality detection and PCR analysis.The results shows that these four ex... |
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| Keywords: | Benzyl chloride method Glass beads method DNA extraction PCR |
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