| 大蒜干腐病病原菌的分离鉴定 |
| |
| 引用本文： | 李旭双,陈典,梁誉,王勇. 大蒜干腐病病原菌的分离鉴定[J]. 中国蔬菜, 2012, 1(20): 88-93 |
| |
| 作者姓名： | 李旭双 陈典 梁誉 王勇 |
| |
| 作者单位： | 东北农业大学园艺学院，黑龙江哈尔滨 150030 |
| |
| 基金项目： | 公益性行业(农业)科研专项(200903018-6) |
| |
| 摘 要： | 以感病的大蒜品系Z-1001鳞茎为材料，经病原菌分离与单孢纯化，获得F、J、H、G 4种纯化菌株。依据病原菌致病性测定，证实F菌株、J菌株为大蒜干腐病主要致病菌株。采用病原菌形态学观察并结合rDNA ITS序列分析，鉴定F菌株为尖孢镰刀菌（Fusarium oxysporum Schlecht），J菌株为层生镰刀菌〔Fusarium proliferatum（Matsush.）Nirenberg〕，说明尖孢镰刀菌（F.?oxysporum Schlecht）和层生镰刀菌〔F.?proliferatum（Matsush.）Nirenberg〕是大蒜干腐病的主要致病菌。
|
| 关 键 词： | 大蒜干腐病 尖孢镰刀菌 层生镰刀菌 形态学 rDNA ITS序列 |
Isolation and Identification of Garlic Dry Rot Disease Pathogen |
| |
| LI Xu-shuang , CHEN Dian , LIANG Yu , WANG Yong. Isolation and Identification of Garlic Dry Rot Disease Pathogen[J]. China Vegetables, 2012, 1(20): 88-93 |
| |
| Authors: | LI Xu-shuang CHEN Dian LIANG Yu WANG Yong |
| |
| Affiliation: | College of Horticulture，Northeast Agricultural University，Harbin 150030，Heilongjiang，China |
| |
| Abstract: | In order to study the pathogens of garlic dry rot disease,this study used the susceptible garlic(Allium sativum L.)strain Z-1001 for pathogen isolation,single-spore purification and pathogenicity determination.The results revealed that the F strain and J strain were main garlic dry rot pathogenic strains.The results of pathogen morphology observation and rDNA ITS sequence analysis showed that F strain was Fusarium oxysporum Schlecht,while J strain was Fusarium proliferatum(Matsush.)Nirenberg,which in turn revealed that F. oxysporum Schlecht and F. proliferatum(Matsush.)Nirenberg were the main pathogens of garlic dry rot disease. |
| |
| Keywords: | Garlic dry rot disease Fusarium oxysporum Schlecht Fusarium proliferatum (Matsush.)Nirenberg Morphology rDNA ITS sequence |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国蔬菜》浏览原始摘要信息 |
|
点击此处可从《中国蔬菜》下载全文 |
