| 抗茶树冰核细菌内生菌的筛选及鉴定 |
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| 引用本文： | 黄晓琴,张丽霞,刘会香,陈宗懋,李多川. 抗茶树冰核细菌内生菌的筛选及鉴定[J]. 茶叶科学, 2015, 35(1): 97-102. DOI: 10.3969/j.issn.1000-369X.2015.01.018 |
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| 作者姓名： | 黄晓琴 张丽霞 刘会香 陈宗懋 李多川 |
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| 作者单位： | 1. 山东农业大学园艺科学与工程学院,山东 泰安 271018; 2. 作物生物学国家重点实验室,山东 泰安 271018; 3. 山东农业大学植保学院,山东 泰安 271018; 4. 中国农业科学院茶叶研究所,浙江 杭州 310008 |
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| 摘 要： | 从茶树内生菌中进行了冰核细菌拮抗菌的筛选,得到菌株Y1,通过对菌株Y1进行形态学观察、生理生化指标测定及16βS rDNA序列测定和序列同源性分析,将菌株Y1鉴定为解淀粉芽孢杆菌（Bacillus amyloliquefaciens）。本研究获得了茶树内生拮抗菌株,明确了菌株Y1的种属,有利于冰核细菌生物防治的开展。
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| 关 键 词： | 茶树 冰核细菌 拮抗菌 内生菌 筛选 鉴定 |
| 收稿时间： | 2014-08-12 |
Screeiningand Identification ofthe Endophytic Bacterial Strain AgainstIce Nucleation Active Bacteria of Tea Plant |
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| HUANG Xiaoqin,ZHANG Lixia,LIU Huixiang,CHEN Zongmao,LI Duochuan. Screeiningand Identification ofthe Endophytic Bacterial Strain AgainstIce Nucleation Active Bacteria of Tea Plant[J]. Journal of Tea Science, 2015, 35(1): 97-102. DOI: 10.3969/j.issn.1000-369X.2015.01.018 |
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| Authors: | HUANG Xiaoqin ZHANG Lixia LIU Huixiang CHEN Zongmao LI Duochuan |
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| Affiliation: | 1. College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China; 2. Shandong Key Laboratory of Crop Biology, Tai’an 271018, China; 3. Department of Plant Pathology, Shandong Agricultural University, Tai’an 271018, China; 4. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China |
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| Abstract: | In order to protect the tea plantagainstfreezing injury, the screeningof antagonistic bacteriaagainstINA bacteria No. 8(Pantoea ananatis)and No.14 (P.agglomerans)isolated fromteaplantwascarriedoutin Shandong Province.The strain Y1,anantagonistic bacteriaagainstINA bacteriawas isolated from theendophyticbacteria strainsof tea plant. Based on themorphological characteristics and 16SrDNA sequence analysis, the strain Y1was identified as theBacillus amyloliquefaciens.Through this study, the endophyticbacteriastrainagainst the ice nucleation bacteriawas obtained and identefied, it will bebeneficial to the development of the biological controlof ice nucleation bacterial. |
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| Keywords: | tea plant ice nucleationactive bacteria antagonisticbacterialstrain endophytic strains screening |
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