| 鹅细小病毒单克隆抗体的制备与鉴定 |
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| 引用本文： | 杜秋明,鲁承,高建伟,赵文婧,申峰勇,孟其麟.鹅细小病毒单克隆抗体的制备与鉴定[J].中国畜牧兽医,2011,38(2):162-164. |
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| 作者姓名： | 杜秋明 鲁承 高建伟 赵文婧 申峰勇 孟其麟 |
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| 作者单位： | (延边大学农学院动物医学系，吉林龙井 133400) |
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| 基金项目： | 吉林省牧业管理局项目(吉牧科字第200902号) |
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| 摘 要： | 为制备鹅细小病毒（goose parvovirus，GPV）单克隆抗体（monoclonal antibody，McAb），利用浓缩的GPV免疫BALB/c小鼠，采用杂交瘤技术，经间接ELISA方法筛选和有限稀释法克隆后获得一株抗GPV单克隆抗体杂交瘤细胞株4B4。经鉴定，其McAb的重链为IgG1亚型，轻链为κ链。ELISA和Western blotting试验结果表明，获得的4B4株McAb可特异性的识别GPV和GPV-VP3蛋白，表明4B4株McAb识别的表位可能位于GPV VP3蛋白的保守区域。为进一步鉴定GPV的表位和GPV诊断试剂的研究奠定了基础。
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| 关 键 词： | 鹅细小病毒 单克隆抗体 VP3蛋白 |
Preparation and Evaluation of Goose Parvovirus Monoclonal Antibody |
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| DU Qiu-ming,LU Cheng,GAO Jian-wei,ZHAO Wen-jing,SHEN Feng-yong,MENG Qi-lin.Preparation and Evaluation of Goose Parvovirus Monoclonal Antibody[J].China Animal Husbandry & Veterinary Medicine,2011,38(2):162-164. |
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| Authors: | DU Qiu-ming LU Cheng GAO Jian-wei ZHAO Wen-jing SHEN Feng-yong MENG Qi-lin |
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| Institution: | (Department of Veterinary Medicine，Agricultural College, Yanbian University，Longjing 133400，China) |
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| Abstract: | In order to prepare the monoclonal antibody of goose parvovirus, BALB/c mice were immunized by concentrated GPV and anti-GPV hybridoma cells（4B4） were obtained by hybridoma technique, indirect ELISA and limiting dilution assay. After identification, the heavy chain of McAb was IgG1 subtype and the light chain was κ chain. The results of ELISA and Western blotting showed that GPV and GPV-VP3 protein could be specially recognized by 4B4 McAb. It indicated that the epitope recognized by 4B4 McAb might be the conservated region of GPV VP3 protein. This research settled the foundation for the characterization of GPV epitope and the investigation of GPV diagnostic reagent. |
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| Keywords: | goose parvovirus monoclonal antibody VP3 protein |
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