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红米色素着生位置及其基因定位
引用本文:王丽华,李杰勤,叶小英,王业文,靳西彪,朱建清. 红米色素着生位置及其基因定位[J]. 种子, 2010, 29(2)
作者姓名:王丽华  李杰勤  叶小英  王业文  靳西彪  朱建清
作者单位:1. 四川农业大学水稻研究所,四川省国际科技合作示范基地,成都,611130;安徽科技学院,安徽,凤阳,233100
2. 安徽科技学院,安徽,凤阳,233100
3. 四川农业大学水稻研究所,四川省国际科技合作示范基地,成都,611130
基金项目:优质抗逆种质资源与半致死突变季引进研究及杂种优势利用 
摘    要:分别对红米和白米做了石蜡切片观察,其色素位于果皮和种皮中;并对红米水稻材料红宝石进行了遗传研究及基因定位。红宝石与白色水稻R272的杂交F1表现为红色,表明色素基因受显性基因控制;同时,其F2群体米色性状遗传分离规律符合3∶1的分离比例,表明红色米皮的性状受1对显性基因控制。利用R272/红宝石的F2群体和微卫星标记,将该基因定位在第7染色体上RM8006和RM21186两个标记之间,其遗传距离分别为4.0cM和2.1cM,在物理图上这两个标记的距离为1.7Mb,并将该基因初步命名为Red。

关 键 词:红米  色素  着生位置  遗传分析  微卫星标记

The Pigment Location and Gene Mapping of Red Rice
WANG Li-hua,LI Jie-qin,YE Xiao-ying,WANG Ye-wen,JIN Xi-biao,ZHU Jian-qing. The Pigment Location and Gene Mapping of Red Rice[J]. Seed, 2010, 29(2)
Authors:WANG Li-hua  LI Jie-qin  YE Xiao-ying  WANG Ye-wen  JIN Xi-biao  ZHU Jian-qing
Affiliation:WANG Li-hua1,2,LI Jie-qin2,YE Xiao-ying1,WANG Ye-wen1,JIN Xi-biao1,ZHU Jian-qing1(1.Rice Research Institute of Sichuan Agricultural University,Chengdu 611130,China,2.Anhui Science , Technology University,Fengyang Anhui 233100,China)
Abstract:It was observed that the pigment was in the pericarp and seed coat with paraffin section. Genetic analysis and gene location were conducted on red rice. All grains of F_1 population derived from red rice and the white rice (R272) were all red rice,and the segregation ratio of the F_2 population was fitted to 3:1 ,indicated that the red pigment trait was controlled by a completely dominant gene. Using the F_2 population from the cross between R 272 and red rice and simple sequence repeats markers, a completely dominant gene temporarily named Red,was located between RM 8006 (the genetic distance was 4.0 cM) and RM 21186 (the genetic distance was 2.1 cM) on the chromosome 7. The distance of two markers was 1.7 Mb on rice physical map.
Keywords:red rice  pigment  location  genetic analysis  microsatellite marker
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