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| HSP70在急性热应激蛋鸭脾脏表达变化的研究 | |
| 引用本文： | 邹丽丽,陶争荣,张存,李国勤,沈军达,任晋东,石放雄,卢立志.HSP70在急性热应激蛋鸭脾脏表达变化的研究[J].中国畜牧杂志,2010,46(3). |
| 作者姓名： | 邹丽丽 陶争荣 张存 李国勤 沈军达 任晋东 石放雄 卢立志 |
| 作者单位： | 1. 浙江省农业科学院畜牧兽医研究所,浙江,杭州,310021;南京农业大学动物科技学院,江苏,南京,210095 2. 浙江省农业科学院畜牧兽医研究所,浙江,杭州,310021 3. 浙江省农业科学院畜牧兽医研究所,浙江,杭州,310021;西北农林科技大学动物科技学院,陕西,杨凌,712100 4. 南京农业大学动物科技学院,江苏,南京,210095 |
| 基金项目： | 现代农业产业技术体系建设专项资金，国家"十一五"科技支撑计划课题，浙江省重大科技攻关项目 |
| 摘 要： | 采用半定量RT-PCR与Western-blotting方法检测相同温度、不同时间(3、5、7h)热应激处理鸭脾脏组织中HSP70基因的表达水平,以研究热应激对HSP70基因表达的影响。结果表明:与对照组相比,热应激组蛋鸭脾脏HSP70 mRNA转录水平在热应激进行到3h时呈现下降趋势,但是差异不显著;而进行到5h时则出现显著(P0.05)的回升;热应激组蛋鸭脾脏HSP70蛋白表达水平在热应激进行到5h时出现显著下降(P0.05),表明急性热应激对蛋鸭脾脏组织HSP70基因表达具有重要影响。 |
| 关 键 词： | 热应激 脾脏 热休克蛋白70 表达 蛋鸭 |
Effects of Acute Heat Stress on the Expression Levels of HSP70 Gene in Duck Spleens |
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| ZOU Li-li,TAO Zheng-rong,ZHANG Cun,LI Guo-qin,SHEN Jun-da,REN Jin-dong,SHI Fang-xiong,LU Li-zhi.Effects of Acute Heat Stress on the Expression Levels of HSP70 Gene in Duck Spleens[J].Chinese Journal of Animal Science,2010,46(3). | |
| Authors: | ZOU Li-li TAO Zheng-rong ZHANG Cun LI Guo-qin SHEN Jun-da REN Jin-dong SHI Fang-xiong LU Li-zhi |
| Institution: | ZOU Li-li1,2,TAO Zheng-rong1,ZHANG Cun1,LI Guo-qin1,SHEN Jun-da1,REN Jin-dong1,3,SHI Fang-xiong2,LU Li-zhi1(1.Institute of Animal Science , Veterinary Medicine,Zhejiang Academy of Agricultural Sciences,Zhejiang Hangzhou 310021,China,2.College of Animal Science , Technology,Nanjing University of Agricultural Sciences,Jiangsu Nanjing 210095,3.College of Animal Science , Technology,Northwestern University of Agriculture , Forestriy Sciences,Shanxi Yangling 712100,China ) |
| Abstract: | In order to research the heat stress's influence on the expression of HSP70,we used the method of RT-PCR and western-blotting to test the expression of HSP70 in the spleen of ducks which were treated with the heat stress for the same temperature but different time(3,5,7 h).The result showed that compared with the control,by 3 h heat stress,the expression of HSP70 mRNA was decreased(P > 0.05),while by 5 h heat stress,the expression of HSP70 mRNA distinctively was elevated(P < 0.05) compared with 3 h heat str... |
| Keywords: | heat stress spleen HSP70 expression laying duck |
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