| 海南乐东哈密瓜粉虱传双生病毒的PCR检测 |
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| 引用本文： | 林韶东,车海彦. 海南乐东哈密瓜粉虱传双生病毒的PCR检测[J]. 热带生物学报, 2013, 4(2): 124-128 |
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| 作者姓名： | 林韶东 车海彦 |
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| 作者单位： | [1]海南大学环境与植物保护学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所,海南海口571101 [3]海南省热带农业有害生物监测与控制重点实验室,海南海口571101 |
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| 基金项目： | 农业部热作病虫害疫情监测与防治项目（13RZBC）; 海南大学211工程建设项目 |
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| 摘 要： | 利用双生病毒简并引物PA/PB,对海南乐东哈密瓜感病植株的4个样品（HA1,HA2,HA3,HA4）进行了PCR检测,其中有3个样品扩增到特异片段,PCR产物经克隆后进行序列测定,特异片断大小均为509bp。通过系统进化分析,表明HA1与SLCPHV-[Taiwan],SLCPHV聚类在一个大的分支上,HA3,HA4与SL-CCNV-[Melon],SLCCNV-[Hn61]聚类在一个大的分支上。结果表明,在海南哈密瓜上可能存在不同种的双生病毒的侵染。
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| 关 键 词： | 哈密瓜 粉虱传双生病毒 海南乐东 |
PCR Detection of Whitefly transmitted Geminivirus of Cucumis melo var. Saccharinus in Ledong, Hainan Province |
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| LIN Shaodong,CHE Haiyan. PCR Detection of Whitefly transmitted Geminivirus of Cucumis melo var. Saccharinus in Ledong, Hainan Province[J]. Journal of Tropical Biology, 2013, 4(2): 124-128 |
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| Authors: | LIN Shaodong CHE Haiyan |
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| Affiliation: | 1. College of Environment and Plant Protection,Hainan University,Haikou 570228,China; 2. Environment and Protection Institute,CATAS,Haikou 571101,China; 3. Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,Haikou 571101,China) |
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| Abstract: | Four samples of diseased Cucumis melo var. Saccharinus showing stunting,small leaf,mosaic symptom were collected in Ledong,Hainan Province,China. PCR product of expected size was amplified from three samples using the begomovirus degenerate primer pair PA/PB,cloned and sequenced. The size of the PCR product was 509 bp. Phylogenetic analysis showed that HA1 clustered with SLCPHV-[Taiwan]and SLCPHV while HA3 and HA4 clustered with SLCCNV-[Melon]and SLCCNV-[Hn61],which indicated that the three samples of C. melo var. Saccharinus were infected likely with different species of geminiviruses. |
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| Keywords: | Cucumis melo var. Saccharinus whitefly-transmitted Geminivirus Ledong Hainan Province |
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