| 土壤酸水解氨基酸和氨基糖态氮测定方法的比较研究 |
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| 引用本文： | 刘延美,刘小虎. 土壤酸水解氨基酸和氨基糖态氮测定方法的比较研究[J]. 吉林农业科学, 2010, 35(2): 19-23 |
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| 作者姓名： | 刘延美 刘小虎 |
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| 作者单位： | 沈阳农业大学土地与环境学院/辽宁省农业资源与环境重点实验室,沈阳,110161 |
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| 基金项目： | 国家自然科学基金项目(40871149) |
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| 摘 要： | 对3种不同土壤(黑土、棕壤、红壤)用6 mol·L-1HCl水解后,分别采用Bremner蒸馏法与色谱法测定土壤中氨基糖态氮、氨基酸态氮的含量。结果表明,对于土壤氨基糖态氮,Bremner蒸馏法和色谱法测定结果和精密度无显著差异,但对于黑土和棕壤中的氨基酸态氮,色谱法的检测更为完全,且有较高的精密度。利用色谱法可实现土壤中各种氨基糖和氨基酸分离和定量化,对深入研究土壤中氮的来源及转化具有十分重要的意义。
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| 关 键 词： | Bremner蒸馏法 色谱法 氨基糖态氮 氨基酸态氮 土壤 |
Comparison of Methods for Determining the Content of Hydrolyzed Amino sugar-N and Amino acid-N in Soil |
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| LIU Yan-mei,LIU Xiao-hu. Comparison of Methods for Determining the Content of Hydrolyzed Amino sugar-N and Amino acid-N in Soil[J]. Journal of Jilin Agricultural Sciences, 2010, 35(2): 19-23 |
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| Authors: | LIU Yan-mei LIU Xiao-hu |
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| Affiliation: | College of Land and Environment Science;Shenyang Agricultural University/Key Laboratory of Agricultural Resource and Environment in Liaoning Province;Shenyang 110161;China |
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| Abstract: | The content of amino sugar-N and amino acid-N in three types of soil(black soil,brown soil and red soil) was determined by Bremner method and chromatography detection after being hydrolyzed in 6 mol.L-1HCl.The results showed that there was no significant difference in both the content and the variation between the two methods for the determination of amino sugar-N in soil.However,the content of amino acid-N in black soil and brown soil detected by liquid chromatography was significantly higher than that by ... |
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| Keywords: | Bremner method Chromatography detection Amino sugar-N Amino acid-N Soil |
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