三叶青种质资源遗传多样性的随机扩增多态性DNA分析

利用RAPD技术对64个三叶青种质资源样本进行遗传多样性分析,从40对引物中筛选出10对引物进行批量PCR实验。结果表明:64个三叶青样本的观测等位基因数(Na)为1.666 7~2.000 0,有效等位基因数(Ne)为1.247 9~1.701 3,Nei's基因多样性指数为0.167 0~0.398 4,Shannon多样性指数(I)为0.262 8~0.583 0,平均多态位点为7.5,平均多态百分数为93.145%;在遗传相似系数0.720 56处,可以将64个三叶青样本分成11大类;在遗传相似系数0.697 04处,则可将64个三叶青样本分成4大类;聚类分析的结果与种源的地理距离存在不一致性,这可能与种质资源库的地形、气候等自然因素有关。表明所测三叶青种质资源遗传多样性丰富,具有一定的开发利用价值,可为合理保护三叶青的基因资源及其遗传改良提供科学依据。

Genetic diversity analysis of Tetrastigma hemsleyanum Diels et Gilg germplasm resources by random amplified polymorphic DNA technique

Genetic diversity of 64 samples of Chinese Tetrastigma hemsleyanum Diels et Gilg germplasm resources was analyzed by random amplified polymorphic DNA (RAPD). 10 pairs of primers were screened from 40 primers for batch PCR experiments. The results showed as follows: The observed number of alleles (Na) in 64 samples was 1.666 7-2.000 0, effective number of alleles (Ne) in 64 samples was 1.247 9-1.701 3, Nei's gene diversity index in 64 samples was 0.167 0-0.398 4, Shannon diversity index (I) in 64 samples was 0.262 8-0.583 0, the average polymorphic loci was 7.5, the average the percentage of polymorphism was 93.145%; 64 samples of Tetrastigma hemsleyanum Diels et Gilg were divided into 11 groups at the similarity of 0.720 56, and they were divided into 4 categories at the similarity of 0.697 04. The result of cluster analysis was not consistent with the geographical distance of the provenance, which might be related to the natural factors such as the topography and climate of the germplasm bank. The results suggested that Tetrastigma hemsleyanum Diels et Gilg germplasm resources were abundant in genetic diversity, and had certain value of exploitation and utilization. The results could also provide scientific basis for reasonable protection of genetic resources and genetic improvement of Tetrastigma hemsleyanum Diels et Gilg.