棘胸蛙白内障病原鉴定及病理组织观察

为了确定棘胸蛙(Quasipaa spinosa)白内障病的病原,进行了病原菌分离、人工感染、分离菌理化特性和16S rDNA基因序列分析。结果表明,从患病棘胸蛙分离出2种致病菌Mm1和Mm2,将其大腿肌肉注射感染健康棘胸蛙,表现出与自然发病蛙相似症状。通过理化特性与16S rDNA基因序列鉴定,分离菌为摩氏摩根菌(Morganella morganii),其对链霉素和庆大霉素高度敏感,对氨苄西林和头孢他啶产生抗药性。病理组织学观察发现,其感染可引起棘胸蛙白内障病,发病棘胸蛙的晶状体、肝、肠和肾损伤严重,出现组织溶解、核溶解等现象,且在肠部出现包涵体。本研究发现摩氏摩根菌可感染棘胸蛙,导致棘胸蛙白内障病,并初步研究了病原菌的生物学特性、病理特征和药物敏感性,以期为该病的诊断和防治提供理论依据。

Pathogen isolation and identification of cataract disease from Chinese spiny frog (Quasipaa spinosa ) and its histological observation

To define the pathogen of cataract disease from Chinese spiny frog (Quasipaa spinosa), the pathogen isolation, artificial infection, physical and chemical characteristics of isolated bacteria and sequence analysis of 16S rDNA were analyzed. The results showed that two pathogens (Mm1 and Mm2) were isolated from the diseased frogs. Healthy Chinese spiny frogs injected the isolated intramuscularly exhibited the similar clinical signs, gross and histopathological lesions to the naturally infected frogs. The isolated strains were identified to be Morganella morganii according to physicochemical properties and the 16S rDNA gene sequence analysis. The strains were sensitive to streptomycin and gentamycin, but resistant to ampicillin and cephalosporin. Histopathological observation found that Morganella morganii infection in Chinese spiny frogs could cause pathological damages, especially in the crystalline lens, liver, intestine, and kidney, accompanied by the emergence of karyolysis, histolysis, and inclusion body in intestine. This study found that Chinese spiny frogs could be infected with Morganella morganii and lead to cataract disease. Biological and pathological characteristics, drug sensitivities were analyzed. These results could provide theoretical basis for the diagnosis and treatment of cataracts disease of Chinese spiny frog.