短季棉早熟性的分子标记及QTL定位

以两个陆地棉品种中棉所36×TM-1的207个F2单株为作图群体,筛选出73个多态性引物,25个SSR标记、35个RAPD标记和13个SRAP标记,构建了第一张以研究短季棉为主的包含43个标记,标记间的最小遗传距离为11.8 cM,最大遗传距离为48.9 cM,总长1174.0 cM的遗传连锁图谱,覆盖棉花基因组总长度的23.48%。检测到与短季棉早熟性状相关的12个QTLs,其中有8个QTLs呈簇分布在LG1连锁群上,找到对表型变异的贡献率在30%以上与全生育期、霜前花率和开花期有关的QTL各1个。

Construction of Molecular Linkage Map and QTL Mapping for Earliness in Short-season Cotton

CCRI 36,a short season cotton variety,and TM-1 were used to create 207 F_2 segregating population in Anyang,Henan.Two parents were used to screen 73 polymorphism markers from 518 pairs of SSR primers,1200 RAPD primers and 153 pairs of SRAP primers.207 F_2 populations were tagged and mapped with 73 polymorphism markers,including 25 SSR,35 RAPD and 13 SRAP markers.43 of the informative loci were used in linkage map construction and were assigned into 5 linkage groups.The linkage map was composed of 9 SSR loci,28 RAPD loci and 6 SRAP loci.The total length was about 1174.0 cM,covering 23.48% of the whole cotton genome.The linkage distance ranged from(11.8) cM to 48.9 cM.The marker number associated with a particular group was from 2 to 23 while 30 loci were not linked to the map.7.16%~39.73% phenotype variance could be explained in the F_2 generation.12 QTLs were examined,among them 8 QTLs were clustered on the first linkage group according to our study.More than 30% phenotype variance in the F_2 segregation population was explained by 3 QTLs relating to the sowing to boll opening period,and the sowing to flowering period and the percent of pre-frost lint,respectively.The linkage map construction for study on short season cotton,QTLs against earliness mapping and clustering haven't been reported before.The results are valuable to further research for earliness MAS of short season cotton.