| 黔产紫苏挥发油提取方法优化及化学成分分析 |
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| 引用本文： | 张敏,周英,骆静,陈琳,俸婷婷.黔产紫苏挥发油提取方法优化及化学成分分析[J].贵州大学学报(农业与生物科学版),2016(5). |
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| 作者姓名： | 张敏 周英 骆静 陈琳 俸婷婷 |
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| 作者单位： | 1. 贵州大学 药学院，贵州 贵阳550025; 贵州省药食同源植物资源开发工程技术研究中心，贵州 贵阳550025; 贵州省药食两用资源应用开发工程实验室，贵州 贵阳550025;2. 贵州大学 药学院,贵州 贵阳,550025 |
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| 基金项目： | 贵州省药食同源资源研究开发科技创新人才团队(黔科合人才团队[2015]4010号)，贵州省药食同源植物资源开发工程技术研究中心(黔科合G字[2015]4001)，贵州省药食两用资源应用开发工程实验室(黔发改投资[2015]542号)，贵州省中药民族药制药工程专业学位研究生工作站(黔教研合JYSZ字[2014]002)。 |
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| 摘 要： | 本文应用水蒸气蒸馏法,通过考察浸泡时间、料液比、提取时间等因素,建立了黔产紫苏挥发油提取方法,并对所提取挥发油进行了化学成分分析,采用峰面积归一化法测定了各组分的相对百分含量。研究结果表明,应用水蒸气蒸馏法,浸泡2 h,料液比1∶10,提取3 h,挥发油提取率最高。经GC-MS分析测定共52种成分,主要成分为戊基-2-呋喃基酮,相对百分含量是47.22%。
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| 关 键 词： | 黔产紫苏 挥发油 化学成分 |
Extraction and analysis of chemical composition of essential oil from Perilla frutescens [ Lamia-les:Lamiaceae] in Guizhou |
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| Abstract: | This paper describes the use of steam distillation to extract essential oil from Perilla frutescens in Guizhou . The optimal extraction method was ascertained through investigating those factors such as solid-liquid ratio, immersion time and steam distillation time. The chemical composition of the essential oil was detected by Gas Chromatography Mass Spec-trometry ( GC-MS) . Then the relative percentage content of each composition was determined by using peak area normali-zation method. The results show that the optimum steam distillation method is solid-liquid ratio of 1 ∶ 10, immersion time of 2 h and steam distillation time of 3 h. GC-MS analysis results show that there are 52 ingredients of compositions in the essential oil from Perilla frutescens. The main ingredient is n-amyl 2-furyl ketone with relative percentage of 47.22%. |
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| Keywords: | Perillae frutescens essential oil GC-MS |
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