AsiaⅠ型口蹄疫病毒灭活疫苗毒株不同宿主系传代中VP1基因的遗传变异研究

口蹄疫病毒结构蛋白VP1参与构成病毒粒子的主要中和抗原位点，是4种结构蛋白中最易发生变异的。在病毒传代过程中，对VP1基因进行遗传变异分析是口蹄疫疫苗研制中不可或缺的环节。为此，作者扩增了经不同宿主系（乳鼠、BHK₂₁细胞）连传不同代次的AsiaⅠ型毒株的VP1基因，并对其进行遗传变异分析，毒株间核苷酸同源性为99.4%~99.8%，推导氨基酸序列同源性为98.6%~100%；制苗毒株经过不同宿主系有限传代后，与传代前的原毒(MF1)相比，VP1基因未发生大的变异，主要抗原位点较稳定，说明以此种方式获得的制苗毒株制备的灭活疫苗是稳定的，适用于该毒株流行区域内相关家畜的免疫预防。

Studies on Genetic Variation of VP1 Genes of Asia I FMDV Inactivated Vaccine Strains from Serial Passages in Different Hosts

The VP1 gene of FMDV contains major antigenic determinant and has the highest mutation rate in four structural proteins.Analysis of genetic variation during the passage is indispensable for vaccine research.This paper was studied on the sequence analysis of the VP1 gene of inactivated vaccine strains of FMDV which was generated in different hosts such as suckling mouse and BHK21 cell.The result demonstrated that the homologies among VP1 gene sequences were 99.4% to 99.8%,and the homologies among the deduced amino acid sequences were 98.6% to 100%.Compared with the MF1,VP1 gene and the main antigenic sites were conserved.So it is stable for producing inactivated vaccine and suitable to immune prevention of related livestock in prevailed area.