SRAP构建玉米杂交种指纹图谱的研究

摘 要：【研究目的】SRAP（相关序列扩增多态性）是近年来发展起来的一种新型的DNA标记技术，本文旨在探讨SRAP技术用于玉米品种鉴定的可行性，并建立相应技术体系。【方法】采用25个引物组合对19个玉米杂交种进行了SRAP扩增。【结果】19个引物组合扩增出了多态性条带，共扩增出152条多态性条带，平均每个引物组合产生8条多态性带，多态性频率从44.4%（me4/em3）～91.7%(me4/em1)。19个引物组合检测到的平均PIC为0.879，而引物组合me4/em1可区分供试的所有19个玉米品种。进一步筛选了5对引物，构建了供试材料的指纹图谱，为SRAP用于玉米鉴定奠定了基础。【结论】SRAP具有很高的分辨率，而且操作简便、稳定可靠，具备了用于作物品种鉴定的条件，用于玉米品种鉴定是可行的，可作为SSR技术的重要补充。

Development of SRAP Fingerprinting in 19 Maize Hybrids

Abstract：【OBJECTIVE】Sequence-related amplified Polymorphism(SRAP) is a fairly new PCR-based molecular marker technique developed recent years. The objective of this study was to discuss the possibility of applying SRAP in maize hybrid identification, and to develop identification system. 【METHOD】Nineteen maize hybrids were analyzed by SRAP technique with 25 pairs of SRAP primers. 【RESULTS】Nineteen pairs out of 25 pairs of SRAP primers were selected, which displayed polymorphism, stable and repeated amplification profiles. A total of 152 polymorphic SRAP bands were detected and one primer pair produced 8 polymorphic bands on average. The ratio of polymorphic bands was from 44.4%（me4/em3）to 91.7%(me4/em1). The Polymorphic Information Content (PIC) for the SRAP varied from 0.752 to 0.922 with an average of 0.879. Prominently, primer pair me4/em1 can distinguish the 19 maize hybrids by itself. Furthermore, 5 primer pairs were selected and fingerprinting of 19 maize hybrids were developed, which would be basement of maize identification by SRAP technique. 【CONCLUSION】The results verified very high discrimination ability of SRAP markers, moreover, considering its simple, stable, co-dominant and well- proportioned distribution, conclusion could be made that SRAP might be a potentially useful marker technique and supplement for SSR in maize hybrid identification.