Modulation of Uptake and Translocation of Iron and Copper from Root to Shoot in Common Bean by Siderophore-Producing Microorganisms |
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| Authors: | Guillermo Carrillo-Castañeda Juana Juárez Muñoz José R. Peralta-Videa Eduardo Gomez Jorge L. Gardea-Torresdey |
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| Affiliation: | 1. Colegio de Postgraduados, Instituto de Recursos Genéticos y Productividad, Especialidad de Genética , Montecillo, México;2. Centro de Investigaciones Forestales, Universidad Autónoma del Estado de Hidalgo , Tulancingo, México;3. Environmental Science and Engineering , The University of Texas at El Paso , El Paso, TX, USA;4. Department of Chemistry , The University of Texas at El Paso , El Paso, TX, USA;5. Environmental Science and Engineering , The University of Texas at El Paso , El Paso, TX, USA;6. Department of Chemistry , The University of Texas at El Paso , El Paso, TX, USA |
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| Abstract: | Microbes have developed high-affinity uptake mechanisms to assimilate iron (Fe) and other metals such as aluminum (Al), gallium (Ga), chromium (Cr), and copper (Cu). Siderophores, which are metal chelating compounds, and membrane receptor proteins are involved in these specialized mechanisms. A few siderophore-producing microorganisms associated with plant roots also influence the uptake of some metals. In this study, the potential microbial-assisted Cu and Fe uptake by Phaseolus vulgaris (common bean) plants was evaluated. Seedlings of cultivated common bean varieties Bayo-INIFAP (B) and Negro-150 (N) and wild types yellowish (WY) and black (WB) were developed in the presence of a Cu and Fe solution and associated with the siderophore-producing microorganisms R. leguminosarumbv. Phaseoli (strains 19, 44, and 46); Pseudomonas fluorescens(strain Avm), and Azospirillum brasilense (strain 154). Seedlings of cultivated variety N and black wild type WB inoculated with the strain CPMex.44 accumulated 71% and 30% more Fe than the un-inoculated plants, respectively; however, the wild black bean accumulated the highest absolute amount of Fe (221.56 mg/kg of dry matter) as compared with the cultivated black variety N (126.16 mg/kg of dry matter) (P < 0.05). In the wild type WY seedlings, the highest Fe accumulation was observed when the seeds were inoculated with the Pseudomonas strain Avm (206 mg/kg of dry matter) (P < 0.05). The interaction of Pseudomonas strain Avm with seedlings of the cultivated B variety and the wild type WB promoted the highest accumulation of Cu (51 and 54 mg/kg of dry matter, respectively), 7 and 14 mg more than in the respective non-inoculated seedlings. No promotion of Fe accumulation was observed in the seedlings of the cultivated B variety and in roots; instead, less Fe was accumulated. The wild type WY did not show any improvement in Cu accumulation. In this study, Rhizobiumstrains promoted Fe but not Cu uptake in P. vulgaris seedlings while Pseudomonas strains promoted the uptake of both Cu and Fe. |
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| Keywords: | plant-microbe interactions metal assimilation metal translocation siderophores |
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