| 化脓隐秘杆菌候选抗原的免疫保护效果测试 |
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| 引用本文： | 徐登峰,余远迪,张素辉,付利芝,许国洋,赵扬扬,沈克飞.化脓隐秘杆菌候选抗原的免疫保护效果测试[J].西北农业学报,2020,30(3):347-353. |
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| 作者姓名： | 徐登峰 余远迪 张素辉 付利芝 许国洋 赵扬扬 沈克飞 |
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| 作者单位： | （1.重庆市畜牧科学院，重庆 402460；2.重庆市兽用生物制品工程技术研究中心，重庆 402460） |
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| 基金项目： | 重庆市基础研究与前沿探索项目（cstc2018jcyjAX0764）；重庆市草食牲畜产业技术体系（2019070501）。 |
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| 摘 要： | 为鉴定化脓隐秘杆菌的新抗原,对被抗血清识别的化脓隐秘杆菌膜蛋白进行质谱分析,采用免疫印迹分析抗原性, ELISA方法测试被免疫小鼠的抗体水平,通过化脓隐秘杆菌攻毒测试免疫保护率。结果显示,铁ABC(ATP结合盒,ATP binding cassette)转运体的底物结合蛋白是化脓隐秘杆菌的1个新保护性抗原,具有良好的抗原性和免疫原性。
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| 关 键 词： | 化脓隐秘杆菌 抗原 抗原性 免疫原性 保护率 |
Immunoprotection Effect Testing of A Candidate Antigen of Trueperella pyogenes |
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| XU Dengfeng,YU Yuandi,ZHANG Suhui,FU Lizhi,XU Guoyang,ZHAO Yangyang and SHEN Kefei.Immunoprotection Effect Testing of A Candidate Antigen of Trueperella pyogenes[J].Acta Agriculturae Boreali-occidentalis Sinica,2020,30(3):347-353. |
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| Authors: | XU Dengfeng YU Yuandi ZHANG Suhui FU Lizhi XU Guoyang ZHAO Yangyang and SHEN Kefei |
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| Abstract: | In order to identify new antigens of Trueperella pyogenes,a membrane protein of T.pyogenes recognized by the antiserum was identified by using mass spectrometry,and its antigenicity was analyzed by immunoblotting.Antibody level of mice immunized by the membrane protein was tested by using ELISA,and the immune protection rate for the immune mice was evaluated by challenged with T.pyogenes.The results showed that substrate binding protein of iron ABC (ATP binding cassette) transporter was a new protective antigen of T.pyogenes,and it had good antigenicity and immunogenicity. |
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| Keywords: | Trueperella pyogenes Antigen Antigenicity Immunogenicity Protective rate |
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