Fecal metabolite analysis of Japanese macaques in Yakushima by LC-MS/MS and
LC-QTOF-MS |
| |
| Authors: | Sayaka TSUCHIDA Takanari HATTORI Akiko SAWADA Koretsugu OGATA Jun WATANABE Kazunari USHIDA |
| |
| Institution: | 1)Chubu University Academy of Emerging Sciences, Kasugai, Aichi 487-8501, Japan;2)Shimadzu Corporation, Kyoto 604-8442, Japan;3)Present address: Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487-8501, Japan;4)Present address: Kyoto University Primate Research Institute, Kanrin 41-2, Inuyama, Aichi 484-8506, Japan |
| |
| Abstract: | We performed a comprehensive fecal metabolite analysis using LC-MS/MS and LC-QTOF-MS approaches as a preliminary study. Feces of Japanese macaques on Yakushima Island were collected from five monkeys at two separate locations. Using the former methodology, 59 substances such as free amino acids, nucleotides, nucleosides and nucleic acid bases, and organic acids in the citrate cycle were quantitatively detected and successfully differentiated in two different monkey groups by the concentrations of nucleic acid metabolites and free amino acids. In the latter, around 12,000 substances were detected both by positive and negative mode in each sample. Differences in signal intensities were observed between two monkey groups in the concentrations of plant secondary metabolites such as cyanogenic glycosides, flavonoids, and phenolics. |
| |
| Keywords: | fecal metabolome liquid chromatography-quadrupole time-of-flight mass spectrometry liquid chromatography-tandem mass spectrometry wild monkey |
|
|
