Anti-lipopolysaccharide antibody administration mitigates ruminal
lipopolysaccharide release and depression of ruminal pH during subacute ruminal acidosis
challenge in Holstein bull cattle |
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Authors: | Hitoshi MIZUGUCHI Tomoki IKEDA Yumi WATANABE Shiro KUSHIBIKI Kentaro IKUTA Yo-Han KIM Shigeru SATO |
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Institution: | 1)DKK-Toa Yamagata Co., Yamagata 996-0053, Japan;2)Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan;3)Institute of Livestock and Grassland Science, NARO, Tsukuba, Ibaraki 305-0901, Japan;4)Awaji Agricultural Technology Center, Minami-Awaji, Hyogo 656-0442, Japan;5)Department of Animal Resources Science, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea |
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Abstract: | The effects of anti-lipopolysaccharide (LPS) antibody on rumen fermentation and LPS activity were investigated during subacute ruminal acidosis (SARA) challenge. Eleven Holstein cattle (164 ± 14 kg) were used in a 3 × 3 Latin square design. Cattle were fed a roughage diet on days −11 to −1 (pre-challenge) and day 2 (post-challenge), and a high-grain diet on days 0 and 1 (SARA challenge). For 14 days, 0-, 2-, or 4-g of anti-LPS antibody was administered once daily through a rumen fistula. Ruminal pH was measured continuously, and rumen fluid and blood samples were collected on days −1, 0, 1, and 2. Significantly lower ruminal LPS activity on day 1 was observed in the 2- and 4-g groups than those in the 0-g group. In addition, significantly higher 1-hr mean ruminal pH on SARA challenge period (days 0 and 1) was identified in the 4-g group than in the 0-g group. However, rumen fermentation measurements (total volatile fatty acid VFA], VFA components, NH3-N and lactic acid) and peripheral blood metabolites (glucose, free fatty acid, beta-hydroxybutyrate, total cholesterol, blood urea nitrogen, aspartate aminotransferase and gamma-glutamyl transferase) were not different among the groups during the experimental periods. Therefore, anti-LPS antibody administration mitigates LPS release and pH depression without the depression of rumen fermentation and peripheral blood metabolites during SARA challenge in Holstein cattle. |
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Keywords: | anti-lipopolysaccharide antibody cattle lipopolysaccharide rumen fermentation subacute ruminal acidosis |
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