| 应用SSR标记技术鉴定玉米杂交种的纯度 |
| |
| 引用本文： | 李素玲,张君捷,柴美清,尚春树.应用SSR标记技术鉴定玉米杂交种的纯度[J].山西农业科学,2007,35(1):36-38. |
| |
| 作者姓名： | 李素玲 张君捷 柴美清 尚春树 |
| |
| 作者单位： | 山西省农业科学院食用菌研究所,山西,太原,030031 |
| |
| 摘 要： | 以利用常规盐溶蛋白电泳技术难以进行纯度鉴定的强盛1号玉米杂交种及相应亲本自交系和6对玉米SSR位点引物为材料,筛选出适合该杂交种纯度鉴定的SSR位点引物,结果表明,bilg 116引物的扩增产物呈现双亲互补的特征谱带,易于区分杂交种和亲本自交系。并通过对单子粒DNA提取等技术环节的改进,建立了一套简单、快速、准确、可靠的种子纯度鉴定方法。
|
| 关 键 词： | 玉米杂交种 DNA提取 纯度 SSR标记技术 |
| 文章编号： | 1002-2481(2007)01-36-03 |
| 修稿时间： | 2006-09-21 |
Application of SSR Marker Technique to Purity Testing of Maize |
| |
| LI Su-ling,ZHANG Jun-jie,CHAI Mei-qing,SHANG Chun-shu.Application of SSR Marker Technique to Purity Testing of Maize[J].Journal of Shanxi Agricultural Sciences,2007,35(1):36-38. |
| |
| Authors: | LI Su-ling ZHANG Jun-jie CHAI Mei-qing SHANG Chun-shu |
| |
| Abstract: | In this study,6 SSR primer pairs were screened to search for the SSR loci that are suitable for testing the purity of Qiangsheng No.1 maize hybrid,which lack a reliable laboratory method for purity testing.The results are as follows: A polymorphic SSR loci,bilg116 has been found that could be applied to test the purity of this hybrid. A procedure featuring inexpensive equipment and ease of use has been set up for testing maize hybrid purity using SSR marker. |
| |
| Keywords: | Maize hybrid DNA extraction Purity SSR marker |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
