Morphological and molecular diversity analysis among the Indian clones of <Emphasis Type="Italic">Sesuvium portulacastrum</Emphasis> L. |
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Authors: | Vinayak H Lokhande Tukaram D Nikam Vikas Y Patade Penna Suprasanna |
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Institution: | (1) Department of Botany, University of Pune, Pune, 411007, India;(2) Functional Plant Biology Section, Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India |
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Abstract: | Sesuvium portulacastrum L. (seapurslane) is a halophyte used as pioneer species in sand dune fixation and stabilization of saline soil. Studies on
the morphological and molecular diversity were carried out for the 14 clones of Sesuvium collected from the different coastal regions of India. Significant differences were observed for morphological traits viz.,
length, width, diameter and area of leaf, internodal distance and stem diameter for different clones when compared with the
clone from Gujarat state (GJ1). A UPGMA dendrogram for morphological traits based on the Pearson’s similarity coefficient
clustered the clones into three groups considering 80% polymorphism as criteria. Molecular diversity among the clones was
studied using Randomly Amplified Polymorphic DNA (RAPD), Internal Transcribed Spacer (ITS) and markers specific to Ac homologous region. Of the total 749 RAPD loci amplified with 70 random primers, 294 were polymorphic with 39.25% diversity.
A phylogenetic tree constructed with UPGMA and SHAN, grouped the clones into three major clades based on RAPD data. The molecular
diversity studied with ITS and markers specific to Ac homologous region revealed 37.50% and 66.66% polymorphism and clustered the clones into three and four clades, respectively.
The genetic diversity analysis revealed wide variations among the S. portulacastrum clones, reflecting a high level of diversity within the species which might be due to anthropogenic impact and geographic
environmental conditions. Further, the various clones from the different eco-geographic coastal localities might have originated
from native places of wild abundance. To the best of our knowledge, this is the first attempt to evaluate both morphological
and genetic diversity among the Sesuvium clones collected from the distant habitats of the coastal regions of the India. |
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Keywords: | Ac homologous region ITS Morphological and molecular diversity RAPD Sesuvium portulacastrum |
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