山羊、水牛感染肝片吸虫后淋巴细胞自然杀伤活性动态变化

自然杀伤 (NK)活性是在细胞水平反映机体天然免疫力的重要指标之一。本实验以SP2 / 0细胞为靶细胞 (即效应细胞 ) ,采用乳酸脱氢酶(LDH)短程释放法测定人工感染肝片吸虫的水牛和山羊的外周血液中淋巴细胞的NK活性动态变化。从测定结果的相对值来看 ,发现山羊在感染肝片吸虫后NK活性有 2次高峰 ,推测与虫体的发育或感染山羊的免疫状态有关 ;2个感染组 (Ⅰ ,2 0 0个囊蚴 /只 ;Ⅱ ,50 0个囊蚴 /只 )的变化趋势表明 ,感染量与NK活性无相关关系。感染水牛的NK值仅略高于对照组 ,无明显的高峰。而绝对值结果又反映出不同动物的NK活性有差异 ,水牛的NK活性比山羊高 ,说明水牛对肝片吸虫的天然免疫力比山羊强

Kinetic development of NK activity of lymphocytes of goats and buffaloes experimentally infected with F.hepatica

The method of“fast releasing of LDH”was used in detecting NK activity of PBLs of goats and buffaloes experimentally infected with Fasciola hepatica.The target cells were SP2/0 cells.The results showed that NK activity of goats had two tops,which were suggested to be related with growth of worms or immune state of animals.Close changes of two groups(each infected with 200 or 500 metacercariaes)showed that there was no correlation between NK activity and infective amount.NK activity of infected buffaloes were higher than that of controls' although there was no significant difference.The results also showed that these two kinds of animals had different NK activity levels both in nature and after being infected.NK activity of buffaloes were higher than that of goats,which indicated that buffaloes were more resistant to F.hepatica than goats.