不同光照条件下大豆幼苗和不同外植体获得愈伤组织中大豆苷元及染料木素的含量

通过对大豆幼苗光照培养和大豆不同外植体的组织培养，测定不同培养条件下大豆幼苗不同部位及不同外植体培养得到的愈伤组织中大豆苷元和染料木素的含量。结果表明：大豆幼苗光照培养30d时根中大豆苷元及染料木素含量分别是相应培养时间无光照条件根中的3.69倍和3.59倍；在光照培养30d真叶中大豆苷元和染料木素的含量也较高，仅次于根，是相同培养时间无光照条件真叶中大豆苷元和染料木素的含量的2倍。不同外植体培养得到的愈伤组织中，真叶培养得到愈伤组织培养60d时大豆苷元及染料木素的含量最高。正常光照条件下的大豆幼苗，其不同部位大豆苷元及染料木素含量高于对应部位得到的愈伤组织中大豆苷元及染料木素的含量。经过90d组织培养，真叶获得的愈伤组织中大豆苷元、染料木素的总含量较最初的外植体中的总含量分别增加了162.53倍和111.71倍。结合不同外植体诱导出的愈伤组织的重量增长情况。在愈伤组织培养90d时，根、子叶节、子叶作为外植体进行愈伤组织培养，生产大豆苷元及染料木素的效果最好。

Content of Daidzein and Genistein in Soybean Seedlings under Different Light Condition and Callus from Different Explants

Daidzein and genistein contents of soybean seedlings uder different light condition and callus from different soybean ex-plants are determined in this experiment.It showed that the contents of daidzein and genistein in roots of soybean seedling treated with light for 30 days are 3.69 and 3.59 times of those without light.Contents of daidzein and genistein in leaves treated with light for 30 days are twice of those without light.The contents of daidzein and genistein in callus from leaves are highest when cu...