燕麦DNA导入普通小麦变异观察及RAPD分子验证

利用花粉管通道将健壮燕麦(Avera sativaL.)的总DNA导入普通小麦宁春4号中,获得12个稳定变异的品系。农艺性状变异观测结果显示多数变异品系的叶面积、穗长、主穗粒数、主穗粒重和千粒重等性状都显著地超过了受体宁春4号小麦;对受体、供体和稳定变异的品系进行RAPD分析,在68个随机引物中有8个引物检测出DNA的多态性,并且在后代基因组中找到了受体没有而供体特有的DNA片段,从DNA分子水平上证明了燕麦DNA可以通过花粉管通道进入受体,并与其基因组DNA整合,在后代中遗传和表达。

Oat (Avena sativa L.) exogenous DNA introduction into common wheat and PAPD analysis

12 variant strains have been developed form pollen tube pathway method by taking oat(Avena sativa L.) and common wheat(Ningchun 4) as donor and recipient.The major agronomic traits of variant strains such as the flagleaf area,spike length,grain number of main spike,grain weight of main spike and weight of thousand grains were significantly exceeded of Ningchun 4.RAPD analysis with 68 random primers was performed.Products of each primer were detected to have polymorphism among oat,Ningchun 4 and variant strains.The specific DNA fragments of donor have been found in genome of progenies,which didn't exist in the original recipient. Oat total DNA fragments can insert into recipient through pollen tube pathway and perform phenotypic expression can be inherited in progenies.This has been directly verified through technique of molecular level of DNA.