| 儿茶素对蝴蝶兰叶片离体培养褐变发生的影响 |
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| 引用本文： | 谭汝芳,周文灵,许传俊,李玲.儿茶素对蝴蝶兰叶片离体培养褐变发生的影响[J].北方园艺,2009(3). |
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| 作者姓名： | 谭汝芳 周文灵 许传俊 李玲 |
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| 作者单位： | 1. 华南师范大学,生命科学学院,广东省植物发育生物工程重点实验室,广东,广州,510631
2. 华南师范大学,生命科学学院,广东省植物发育生物工程重点实验室,广东,广州,510631;福建省亚热带植物研究所,福建,厦门,361006 |
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| 摘 要： | 用0.3 g/L儿茶素处理蝴蝶兰叶片外植体,在MS+3 mg/L 6-BA(pH 5.8)培养基上培养4 d,外植体褐变率高于对照227.6%.用香草醛-盐酸溶液染色反应法发现鞣质分布在维管束和细胞间隙.叶片剪切的外植体(培养0 d)鞣质含量较高,培养4 d降低,8 d后再升高.儿茶素处理的外植体鞣质含量明显高于对照.在培养期间,儿茶素处理的外植体PPO、POD和PAL活性高于对照外植体,其中POD活性在第8天达到最大值,PPO和PAL活性在培养第12天达到高峰.
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| 关 键 词： | 儿茶素 蝴蝶兰 叶片外植体 褐变 鞣质 |
Effect of Catechin on Browning of Leaf Explants in Tissue Culture of Phalaenopsis sp. |
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| TAN Ru-fang,ZHOU Wen-ling,XU Chuan-jun,LI Ling.Effect of Catechin on Browning of Leaf Explants in Tissue Culture of Phalaenopsis sp.[J].Northern Horticulture,2009(3). |
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| Authors: | TAN Ru-fang ZHOU Wen-ling XU Chuan-jun LI Ling |
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| Institution: | 1.College of Life Science;Guangdong Key Laboratory of Biotechnology for Plant Development;South China Normal University;Guangzhou;Guandong 510631;China;2.Fujian Institute of Subtropical Botany;Xiamen;Fujian 316006;China |
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| Abstract: | The browning percentage of leaf explants of Phalaenopsis sp.,treated with 0.30 mg/L of catechin and cultured in MS medium containing 3 mg/L 6-BA(pH 5.8) for first four days,were higher 227.6% than that of the controls.8%(w/v) vanillin-hydrochloric acid assay confirmed that tannins were distributed in intracellular fractions and vascular bundle of leaf explants.Cutting of leaf explants caused more accumulation of tannin in them cultured 0 day.Tannin contents reduced in the first four days,and then increased ... |
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| Keywords: | Catechin Phalaenopsis sp Leaf explants Browning Tannin |
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