温度和乌苯苷对延边黄牛卵母细胞Na/K ATP酶活性的影响

通过研究温度和不同分级对延边黄牛卵母细胞Na/K ATP酶活性的影响,以及对其特异性抑制剂乌苯苷的敏感浓度,对延边黄牛卵母细胞Na/K ATP酶做初步探究,为进一步研究Na/K ATP酶活性与卵母细胞体外成熟的相关性打下良好基础.结果表明,延边黄牛卵母细胞的Na/K ATP酶活性在50℃达到最大值,在反应温度低于或高于50℃时酶的活性均降低,且在高于50℃时酶活急剧降低.乌苯苷浓度在2 mmol/L时孤雌卵母细胞完全停止卵裂.A级卵母细胞,裸卵和过成熟卵母细胞三者的Na/K ATP酶活性在统计学上差异不显著,但裸卵的Na/K ATP酶活性在数值上略高于其它两组.因此,延边黄牛卵母细胞的Na/K ATP酶的最适反应温度为50℃,其活力能够被2 mmol/L乌苯苷完全抑制,不同质量卵母细胞的Na/K ATP酶活性中,裸卵的Na/K ATP酶活性最高.

Effect of temperature and ouabain on the activity of Na/K ATPase of oocyte in Yanbian Yellow Cattle

Stadied the character of oocytes of Yanbian Yellow Cattle by researching the effect of temperature on oocytes of Yanbian Yellow Cattle and the max concentration of ouabain,the specific inhibitor of Na/K ATPase,and the effect of granular cell on the active of Na/K ATPase,which are the great foundation of the further study about the relationship between the active of Na/K ATPase and the vitro mature of oocytes of Yanbian Yellow Cattle.The results showed that the active of Na/K ATPase reached the max value at 50 ℃,and the active degraded below or over 50 ℃.The partheno oocytes cann＇t cleavage completely in present of ouabain at 2 mmol/L.The active of Na/K ATPase in grade A group,ova nuda group and hyper-mature group were detected,there were no significant difference among the three groups tested,but the value of ova nuda group was the highest.The conclusions were that the optimal react temperature of Na/K ATPase of the oocytes of Yanbian Yellow Cattle was 50 ℃ and the max suppressible concentration of ouabain was 2 mmol/L.The active of Na/K ATPase in ova nuda group was the highest.