Characterization of monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (PSGL-1) |
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| Authors: | Umeki Saori Suzuki Ryoichi Shimojima Masayuki Ema Yasuo Yanase Takuma Iwata Hiroyuki Okuda Masaru Mizuno Takuya |
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| Affiliation: | Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. |
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| Abstract: | Thirteen different monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (cPSGL-1) were obtained by immunization of rats with cells of a canine lymphoma cell line (Ema). O-sialoglycoprotein endopeptidase treatment of Ema cells showed that all of these antibodies recognized O-glycosylated peptides of canine PSGL-1. Experiments using deletion or point mutants of cPSGL-1 indicated that these antibodies could be categorized into several groups based on their cPSGL-1 recognition characteristics. These anti-cPSGL-1 monoclonal antibodies will be useful for analysis of the canine P-selectin and PSGL-1 system. |
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