| 用高效液相色谱法检测底泥环境中孔雀石绿的方法 |
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| 引用本文： | 岑剑伟,杨贤庆,李来好,孙满义,郝淑贤,刁石强,戚勃,马海霞. 用高效液相色谱法检测底泥环境中孔雀石绿的方法[J]. 大连水产学院学报, 2009, 24(6): 568-573 |
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| 作者姓名： | 岑剑伟 杨贤庆 李来好 孙满义 郝淑贤 刁石强 戚勃 马海霞 |
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| 作者单位： | 1. 中国水产科学研究院,南海水产研究所,广东,广州,510300
2. 中国水产科学研究院,南海水产研究所,广东,广州,510300;上海海洋大学,水产与生命学院,上海,201306 |
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| 基金项目： | 广东省省级财政专项资金资助项目 |
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| 摘 要： | 研究了用高效液相荧光法检测养殖池塘底泥中孔雀石绿的方法，比较了不同提取液对孔雀石绿的提取效果，针对相应的提取液用正交试验建立最佳提取方案，采用硼氢化钾将孔雀石绿还原成隐色孔雀石绿后用高效液相色谱荧光检测器进行定量分析。结果表明：前处理中采用甲醇和二氯甲烷混合提取比较简单，最适宜提取方案是提取液用量为20mL，震荡提取5min，重复提取2次；硼氢化钾还原效果良好，用量2mg即能将该体系中的孔雀石绿完全还原。本方法在0．5—800ng／mL范围内具有很好的线性，孔雀石绿总量最低检出限为0．36μg/kg。孔雀石绿和隐色孔雀石绿的加标回收率分别为82．4％～84．8％、88．1％～92．3％，相对标准偏差为3．16％～4．74％、1．91％～4．11％。
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| 关 键 词： | 孔雀石绿 养殖池塘底泥 硼氢化钾 高效液相 荧光检测器 |
Determination of malachite green in the sediments from aquaculture environment by HPLC with fluorescence detector |
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| CEN Jian-wei,YANG Xian-qing,LI Lai-hao,SUN Man-yi,HAO Shu-xian,DIAO Shi-qiang,QI Bo,MA Hai-xia. Determination of malachite green in the sediments from aquaculture environment by HPLC with fluorescence detector[J]. Journal of Dalian Fisheries University, 2009, 24(6): 568-573 |
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| Authors: | CEN Jian-wei YANG Xian-qing LI Lai-hao SUN Man-yi HAO Shu-xian DIAO Shi-qiang QI Bo MA Hai-xia |
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| Affiliation: | CEN Jian-wei1,YANG Xian-qing1,LI Lai-hao1,SUN Man-yi1,2,HAO Shu-xian1,DIAO Shi-qiang1,QI Bo1,MA Hai-xia1(1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China,2.College of Fisheries , Life Science,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | A method to determine malachite green(MG) in sediments from aquaculture environment was studied by high performance liquid chromatography(HPLC) with fluorescence detector.Different extraction solvents were compared and the optimum extract condition was confirmed by orthogonal test.The results showed that the mixture of methanol and dichloromethane was comparably convenient in the extraction assay.The optimum extraction condition was as the following: extracting for 5 min with 20 mL extraction solvent and re... |
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| Keywords: | malachite green sediment KBH4 HPLC fluorescence detector |
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