| γ-射线对苹果和梨离体叶片不定梢再生及芽生长的影响 |
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| 引用本文： | 孙清荣,李林光,樊圣华,刘鹏,杨建明. γ-射线对苹果和梨离体叶片不定梢再生及芽生长的影响[J]. 园艺学报, 2004, 31(2): 217-220 |
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| 作者姓名： | 孙清荣 李林光 樊圣华 刘鹏 杨建明 |
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| 作者单位： | (山东省果树研究所，泰安 271000) |
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| 基金项目： | 国家科技攻关项目(2002BA515B08)，山东省优秀中青年科学家科研奖励基金项目(01DS48) |
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| 摘 要： | 用γ-射线照射苹果和梨的无根无菌苗，研究其对叶片不定梢再生及芽生长的影响。对照射最敏感的器官是叶片，其次是未萌发的侧芽，第三是正在生长的顶芽。叶片不定梢再生的半致死剂量为20~30 Gy，苹果侧芽萌发的半致死剂量为30 Gy，梨为30 Gy以上。照射剂量在10—30 Gy时，抑制顶芽的伸长生长，但不引起致死或其他不良生长症状。
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| 关 键 词： | 苹果 梨 叶片 芽 γ-射线 组织培养 |
| 文章编号： | 0513-353X(2004)02-0217-04 |
| 收稿时间： | 2003-04-07 |
The Effect of Gamma Irradiation on Shoot Regeneration from Leaf and Bud Growth of Pear and Apple in Vitro |
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| Sun Qingrong,Li Linguang,Fan Shenghua,Liu Peng,and Yang Jianming. The Effect of Gamma Irradiation on Shoot Regeneration from Leaf and Bud Growth of Pear and Apple in Vitro[J]. Acta Horticulturae Sinica, 2004, 31(2): 217-220 |
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| Authors: | Sun Qingrong Li Linguang Fan Shenghua Liu Peng and Yang Jianming |
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| Affiliation: | (Shandong Institute of Pomology，Tai'an 271000，China) |
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| Abstract: | The shoots of pear and apple in vitro were irradiated by gamma rays． The effects of gamma irradiation on shoot regeneration from leaf and bud growth were studied． The organ which is the most sensitive to γ-rays was leaf，second was unbudding lateral bud， the third was growing shoot tip． The LD50(50% decrease of regeneration)of leaf was genotype-dependent and was between 20 and 30 grays．The LD50 of lateral bud also was genotype-dependent and was 30 grays or above 30 grays． Doses of iradiation were between 10 and 30 grays，the elongation growth of shoot tip was inhibited， but no lethality or other symptoms．Key words： |
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| Keywords: | Apple Pear Leaf Bud γ-rays Tissue culture |
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